GADD45A ELISA 试剂盒
Quick Overview for GADD45A ELISA 试剂盒 (ABIN2043599)
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检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.15 ng/mL
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灵敏度
- < 0.062 ng/mL
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Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) ELISA Kit
GADD45A 适用: 人 Colorimetric Sandwich ELISA 25 pg/mL - 1600 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) ELISA KitGADD45A 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) ELISA KitGADD45A 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))
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别名
- Growth Arrest And DNA Damage Inducible Protein alpha (GADD45a)
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途径
- p53 Pathway, Cell Division Cycle
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