EGLN1 ELISA 试剂盒
This 小鼠 EGLN1 ELISA Kit specifically detects EGLN1
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产品编号 ABIN2043387
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for EGLN1 ELISA 试剂盒 (ABIN2043387)
抗原
See all EGLN1 products
EGLN1
(Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))
适用
小鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.31-20 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.31 ng/mL
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灵敏度
- < 0.108 ng/mL
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板类型
- Pre-coated
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实验流程
- Egl Nine Homolog 1 (EGLN1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Egl Nine Homolog 1 (EGLN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Egl Nine Homolog 1 (EGLN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Egl Nine Homolog 1 (EGLN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Egl Nine Homolog 1 (EGLN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))
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别名
- Egl Nine Homolog 1 (EGLN1)
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途径
- cAMP Metabolic Process, Warburg Effect
抗原 See all EGLN1 products
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