EGLN1 ELISA 试剂盒
This 小鼠 EGLN1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 EGLN1.
Quick Overview for EGLN1 ELISA 试剂盒 (ABIN2043387)
抗原
See all EGLN1 products
EGLN1
(Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))
适用
小鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.31-20 ng/mL
应用范围
ELISA
-
-
最低检测浓度
- 0.31 ng/mL
-
灵敏度
- < 0.108 ng/mL
-
-
-
板类型
- Pre-coated
-
实验流程
- Egl Nine Homolog 1 (EGLN1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Egl Nine Homolog 1 (EGLN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Egl Nine Homolog 1 (EGLN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Egl Nine Homolog 1 (EGLN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Egl Nine Homolog 1 (EGLN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
-
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C/-20 °C
-
-
- EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))
-
别名
- Egl Nine Homolog 1 (EGLN1)
-
途径
- cAMP Metabolic Process, Warburg Effect
抗原 See all EGLN1 products
-
You are here:
