RAD50 ELISA 试剂盒
This 人 RAD50 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 RAD50.
Quick Overview for RAD50 ELISA 试剂盒 (ABIN2043361)
抗原
See all RAD50 ELISA试剂盒
RAD50
(RAD50 Homolog (S. Cerevisiae) (RAD50))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.15-10 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.15 ng/mL
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品牌
- BioAssay™
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灵敏度
- < 0.057 ng/mL
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RAD50 Homolog (S. Cerevisiae) (RAD50) ELISA Kit
RAD50 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
RAD50 Homolog (S. Cerevisiae) (RAD50) ELISA KitRAD50 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- DNA Repair Protein RAD50 (RAD50) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNA Repair Protein RAD50 (RAD50). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DNA Repair Protein RAD50 (RAD50). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DNA Repair Protein RAD50 (RAD50), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of DNA Repair Protein RAD50 (RAD50) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))
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别名
- DNA Repair Protein RAD50 (RAD50)
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途径
- DNA Damage Repair, Protein targeting to Nucleus
抗原 See all RAD50 ELISA试剂盒
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