C3 ELISA 试剂盒
Quick Overview for C3 ELISA 试剂盒 (ABIN2043197)
抗原
See all C3 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 31.25 ng/mL
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灵敏度
- < 11.2 ng/mL
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板类型
- Pre-coated
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实验流程
- Complement Component 3 (C3) BioAssay-ELISA Kit (Chicken) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 3 (C3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 3 (C3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 3 (C3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Complement Component 3 (C3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- C3 (Complement Component 3 (C3))
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途径
- Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling
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