BCL2L1 ELISA 试剂盒
Quick Overview for BCL2L1 ELISA 试剂盒 (ABIN2042991)
抗原
See all BCL2L1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 7.81 pg/mL
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灵敏度
- < 3.3 pg/mL
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BCL2-Like 1 (BCL2L1) ELISA Kit
BCL2L1 适用: 小鼠 Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Cell Lysate, Tissue Homogenate
BCL2-Like 1 (BCL2L1) ELISA KitBCL2L1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
BCL2-Like 1 (BCL2L1) ELISA KitBCL2L1 适用: 大鼠 Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
BCL2-Like 1 (BCL2L1) ELISA KitBCL2L1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- BCL2L1 (BCL2-Like 1 (BCL2L1))
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别名
- B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L)
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途径
- Apoptosis, Negative Regulation of intrinsic apoptotic Signaling
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