C19ORF80 ELISA 试剂盒
Quick Overview for C19ORF80 ELISA 试剂盒 (ABIN2042857)
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检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.15 ng/mL
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灵敏度
- < 0.054 ng/mL
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Chromosome 19 Open Reading Frame 80 (C19ORF80) ELISA Kit
C19ORF80 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chromosome 19 Open Reading Frame 80 (C19ORF80) ELISA KitC19ORF80 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Chromosome 19 Open Reading Frame 80 (C19ORF80) ELISA KitSmall Sample C19ORF80 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chromosome 19 Open Reading Frame 80 (C19ORF80) ELISA KitC19ORF80 适用: 大鼠 Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Angiopoietin Like Protein 8 (ANGPTL8) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiopoietin Like Protein 8 (ANGPTL8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiopoietin Like Protein 8 (ANGPTL8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiopoietin Like Protein 8 (ANGPTL8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Angiopoietin Like Protein 8 (ANGPTL8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- C19ORF80 (Chromosome 19 Open Reading Frame 80 (C19ORF80))
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别名
- Angiopoietin Like Protein 8 (ANGPTL8)
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