sFAS/Apo-1 ELISA 试剂盒
人 sFAS/Apo-1 ELISA Kit, Colorimetric is an assay for quantification of 人 sFAS/Apo-1.
产品编号 ABIN2042662
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
Quick Overview for sFAS/Apo-1 ELISA 试剂盒 (ABIN2042662)
抗原
See all sFAS/Apo-1 (sFAS) products
sFAS/Apo-1 (sFAS)
(Soluble Factor-Related Apoptosis (sFAS))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
31.2-2000 pg/mL
应用范围
ELISA
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最低检测浓度
- 31.2 pg/mL
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 3 pg/mL
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组件
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96-well plate pre-coated with anti-sFAS, Human
Lyophilized recombinant sFAS, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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板类型
- Pre-coated
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实验流程
- Sandwich ELISA quantitative detection kit for Human Soluble FAS in cell culture supernates, serum and plasma (heparin, EDTA, citrate).Fas, also known as APO-1, CD95 and TNFRSF6, is a member of the nerve growth factor (NGF)/tumour necrosis factor (TNF) receptor superfamily and mediates apoptosis.1 The nucleotide sequence of the cDNAs reveales that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40.2 The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 is previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The APO-1 antigen is found to be a membrane glycoprotein of 48kD.3 Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance.4 Fas can play a role as an inducer of both neurite growth in vitro and accelerates recovery after nerve injury in vivo.5 The FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis.6
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- sFAS/Apo-1 (sFAS) (Soluble Factor-Related Apoptosis (sFAS))
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基因ID
- 355
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UniProt
- P25445
抗原 See all sFAS/Apo-1 (sFAS) products
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