sFAS/Apo-1 ELISA 试剂盒
This Colorimetric ELISA kit is designed for the quantitative measurement of 小鼠 sFAS/Apo-1.
产品编号 ABIN2042663
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Quick Overview for sFAS/Apo-1 ELISA 试剂盒 (ABIN2042663)
抗原
See all sFAS/Apo-1 (sFAS) products
sFAS/Apo-1 (sFAS)
(Soluble Factor-Related Apoptosis (sFAS))
适用
小鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
31.2-2000 pg/mL
应用范围
ELISA
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最低检测浓度
- 31.2 pg/mL
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 3 pg/mL
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组件
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96-well plate pre-coated with anti-sFAS, Mouse
Lyophilized recombinant sFAS, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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板类型
- Pre-coated
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实验流程
- Sandwich ELISA quantitative detection kit for Mouse Soluble FAS in cell culture supernates, serum and plasma (heparin, EDTA, citrate).Fas, also known as APO-1, CD95 and TNFRSF6, is a member of the nerve growth factor (NGF)/tumour necrosis factor (TNF) receptor superfamily and mediates apoptosis.1 The nucleotide sequence of the cDNAs reveales that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40.2 The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 is previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The APO-1 antigen is found to be a membrane glycoprotein of 48kD.3 Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance.4 Fas can play a role as an inducer of both neurite growth in vitro and accelerates recovery after nerve injury in vivo.5
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- sFAS/Apo-1 (sFAS) (Soluble Factor-Related Apoptosis (sFAS))
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基因ID
- 14102
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UniProt
- Q8C350
抗原 See all sFAS/Apo-1 (sFAS) products
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