PLGF ELISA 试剂盒
Quick Overview for PLGF ELISA 试剂盒 (ABIN2042638)
抗原
See all PLGF (PGF) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 15.6 pg/mL
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交叉反应 (详细)
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 1 pg/mL
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组件
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96-well plate pre-coated with anti-PLGF, Human
Lyophilized recombinant PLGF, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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板类型
- Pre-coated
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实验流程
- Sandwich ELISA quantitative detection kit for Human Placental Growth Factor in cell culture supernates, serum, plasma (heparin, EDTA, citrate) and urine.The protein, also called PGF, is 149 amino acids long and shares 53% identity with the platelet-derived growth factor-like region of human VPF. And the N-glycosylated PLGF protein is secreted into the medium and that it functions as a dimer1. The PLGF gene is mapped to 14q24-q31. There are 3 isoforms of PGF, designated PGF1, PGF2, and PGF3. Only PGF2 is able to bind heparin. Additionally, PGF regulates inter- and intramolecular cross-talk between the VEGF receptor tyrosine kinases FLT1 and FLK12. It also can stimulate angiogenesis and collateral growth in ischemic heart and limb with at least a comparable efficiency to VEGF3.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- PLGF (PGF) (Placenta Growth Factor (PGF))
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别名
- PLGF
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基因ID
- 5228
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UniProt
- P49763
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途径
- VEGFR1 Specific Signals
抗原 See all PLGF (PGF) ELISA试剂盒
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