PLGF ELISA 试剂盒
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- 抗原 See all PLGF (PGF) ELISA试剂盒
- PLGF (PGF) (Placenta Growth Factor (PGF))
- 抗原表位
- AA 21-149
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6-1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Placental Growth Factor
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate), Urine
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: A21-R149 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <1pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: E.coli
Immunogen sequence: A21-R149 - Featured
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- Top Product
- Discover our top product PGF ELISA Kit
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Placenta Growth Factor (PGF) ELISA Kit
PGF 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Placenta Growth Factor (PGF) ELISA KitPGF 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Placenta Growth Factor (PGF) ELISA KitPGF 适用: 大鼠 Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Placenta Growth Factor (PGF) ELISA KitPGF 适用: Pig Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Placenta Growth Factor (PGF) ELISA KitPGF 适用: Cow Colorimetric Sandwich ELISA 15.6 pg/M - 1000 pg/M Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Placenta Growth Factor (PGF) ELISA KitPGF 适用: 小鼠 Colorimetric Sandwich ELISA 1-400 pg/mL Cell Culture Supernatant, Plasma, Serum
Placenta Growth Factor (PGF) ELISA KitPGF 适用: 小鼠 AA 24-158 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Placenta Growth Factor (PGF) ELISA KitPGF 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum, Urine
Placenta Growth Factor (PGF) ELISA KitPGF 适用: 小鼠 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Urine
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.
- 板类型
- Pre-coated
- 实验流程
- human PLGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PLGF has been precoated onto 96-well plates. Standards(E.coli, A21-R149) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PLGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PLGF amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human PLGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA, citrate) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human PLGF standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 52, Standard deviation: 3.28, CV(%): 6.3
- Sample 2: n=16, Mean(pg/ml): 146, Standard deviation: 8.47, CV(%): 5.8
- Sample 3: n=16, Mean(pg/ml): 526, Standard deviation: 39.5, CV(%): 7.5,
- Sample 1: n=24, Mean(pg/ml): 45, Standard deviation: 3.24, CV(%): 7.2
- Sample 2: n=24, Mean(pg/ml): 165, Standard deviation: 10.9, CV(%): 6.6
- Sample 3: n=24, Mean(pg/ml): 583, Standard deviation: 49, CV(%): 8.4
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "Angiogenic Capacity of Periodontal Ligament Stem Cells Pretreated with Deferoxamine and/or Fibroblast Growth Factor-2." in: PLoS ONE, Vol. 11, Issue 12, pp. e0167807, (2017) (PubMed).
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: "Angiogenic Capacity of Periodontal Ligament Stem Cells Pretreated with Deferoxamine and/or Fibroblast Growth Factor-2." in: PLoS ONE, Vol. 11, Issue 12, pp. e0167807, (2017) (PubMed).
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- 抗原 See all PLGF (PGF) ELISA试剂盒
- PLGF (PGF) (Placenta Growth Factor (PGF))
- 别名
- PGF (PGF 产品)
- 别名
- D12S1900 ELISA Kit, PGFL ELISA Kit, PLGF ELISA Kit, PlGF-2 ELISA Kit, SHGC-10760 ELISA Kit, AI854365 ELISA Kit, PIGF ELISA Kit, Plgf ELISA Kit, PlGF ELISA Kit, placental growth factor ELISA Kit, placenta growth factor ELISA Kit, PGF ELISA Kit, Pgf ELISA Kit, PLGF ELISA Kit
- 背景
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Protein Function: Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth. .
Background: The protein, also called PGF, is 149 amino acids long and shares 53 % identity with the platelet-derived growth factor-like region of human VPF. And the N-glycosylated PLGF protein is secreted into the medium and that it functions as a dimer1. The PLGF gene is mapped to 14q24-q31. There are 3 isoforms of PGF, designated PGF1, PGF2, and PGF3. Only PGF2 is able to bind heparin. Additionally, PGF regulates inter- and intramolecular cross-talk between the VEGF receptor tyrosine kinases FLT1 and FLK12. It also can stimulate angiogenesis and collateral growth in ischemic heart and limb with at least a comparable efficiency to VEGF3.
Synonyms: Placenta growth factor,PlGF,PGF,PGFL, PLGF,
Full Gene Name: Placenta growth factor
Cellular Localisation: Secreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin. - 基因ID
- 5228
- UniProt
- P49763
- 途径
- VEGFR1 Specific Signals
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