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CD31 ELISA 试剂盒

This Colorimetric ELISA kit is designed for the quantitative measurement of 小鼠 CD31.
产品编号 ABIN2042636
发货至: 中国
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Quick Overview for CD31 ELISA 试剂盒 (ABIN2042636)

抗原

See all CD31 (PECAM1) ELISA试剂盒
CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

6-10000 pg/mL

应用范围

ELISA
  • 最低检测浓度

    6 pg/mL

    交叉反应 (详细)

    There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    96-well plate pre-coated with anti-PECAM-1/CD31, Mouse
    Lyophilized recombinant PECAM-1/CD31, Mouse standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • 板类型

    Pre-coated

    实验流程

    Sandwich ELISA quantitative detection kit for Mouse PECAM-1/CD31 in cell culture supernates, serum and plasma (heparin, EDTA).PECAM-1 is an important target GP in DITP.1 Platelet/endothelial cell adhesion molecule-1 (PECAM-1) is a 130kD member of the Ig gene superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, and selective T-cell subsets.2 Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) is a member of the immunoglobulin (Ig) superfamily that has distinctive features of an immunoreceptor based upon its genomic structure and the presence of intrinsic immunoreceptor tyrosine inhibitory motifs (ITIMs) in its ligand binding polypeptide.3 PECAM-1 has recently been shown to contain functional immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within its cytoplasmic domain, and co-ligation of PECAM-1 with the T-cell antigen receptor (TCR) results in tyrosine phosphorylation of PECAM-1, recruitment of Src homology 2 domain-containing protein tyrosine phosphatase-2 (SHP-2), and attenuation of TCR-mediated cellular signaling.4 The standard used in this kit is the product of gene recombinant expression (extracellular part), consisting of 563 amino acids (E28-K590) with the molecular weight of 65.5kD. After glycosylating, its molecular weight changes to 91-100kD.

    限制

    仅限研究用
  • 储存条件

    4 °C/-20 °C
  • 抗原 See all CD31 (PECAM1) ELISA试剂盒

    CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

    别名

    PECAM-1/CD31

    基因ID

    18613

    UniProt

    Q08481

    途径

    Regulation of Actin Filament Polymerization
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