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CD31 ELISA 试剂盒

人 CD31 ELISA Kit, Colorimetric is an assay for quantification of 人 CD31.
产品编号 ABIN2042635
发货至: 中国
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Quick Overview for CD31 ELISA 试剂盒 (ABIN2042635)

抗原

See all CD31 (PECAM1) ELISA试剂盒
CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

适用

  • 12
  • 10
  • 5
  • 5
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

6-10000 pg/mL

应用范围

ELISA
  • 最低检测浓度

    6 pg/mL

    交叉反应 (详细)

    There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    96-well plate pre-coated with anti-PECAM-1/CD31, Human
    Lyophilized recombinant PECAM-1/CD31, Human standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • 板类型

    Pre-coated

    实验流程

    Sandwich ELISA quantitative detection kit for Human PECAM-1/CD31 in cell culture supernates, serum and plasma (heparin, EDTA).Human platelet/endothelial cell adhesion molecule-1 (PECAM1), an important member of the immunoglobulin gene superfamily, is widely distributed on cells of the vascular system and mediates cellular interactions through both homophilic and heterophilic adhesive mechanisms. The function of PECAM1 in vitro has begun to be understood, but its function in vivo is yet to be established.1 The PECAM1 locus is on the long arm of chromosome 17, in the region q23-qter. To confirm this observation and obtain a more precise localization of the PECAM1 locus, fluorescence in situ hybridization was conducted. Together our data allowed assignment of the PECAM1 locus to the region 17q23.2 To examine the functional role of PECAM-1 in regulating platelet-collagen interactions, 2 different approaches were applied using recombinant human PECAM-1-immunoglobulin chimeras and platelets derived from PECAM-1-deficient mice.3 PECAM-1, an integral membrane protein with an essential role in TEM, is found in this compartment and constitutively recycles evenly along endothelial cell borders.4 The standard used in this kit is the product of gene recombinant expression (extracellular part), consisting of 574 amino acids (Q28-K601) with the molecular weight of 64.5kD. After glycosylating, its molecular weight changes to 90-100kD.

    限制

    仅限研究用
  • 储存条件

    4 °C/-20 °C
  • 抗原 See all CD31 (PECAM1) ELISA试剂盒

    CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

    别名

    PECAM-1/CD31

    基因ID

    5175

    UniProt

    P16284

    途径

    Regulation of Actin Filament Polymerization
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