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MMP3 ELISA 试剂盒

This 人 MMP3 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 MMP3.
产品编号 ABIN2042605
发货至: 中国

Quick Overview for MMP3 ELISA 试剂盒 (ABIN2042605)

抗原

See all MMP3 ELISA试剂盒
MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))

适用

  • 12
  • 12
  • 6
  • 6
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  • 3
  • 2
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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

6-10000 pg/mL

应用范围

ELISA
  • 最低检测浓度

    6 pg/mL

    交叉反应 (详细)

    There is cross-reactivates with MMP-10 approximately 2 % , and no detectable cross-reactivity with other MMPs.

    灵敏度

    < 10 pg/mL

    组件

    96-well plate pre-coated with anti-MMP-3, Human
    Lyophilized recombinant MMP-3, Human standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • 板类型

    Pre-coated

    实验流程

    Sandwich ELISA quantitative detection kit for Human MMP-3 in cell culture supernates, serum and plasma (heparin).Matrix metalloproteinase-3 (MMP-3) also called stromelysin or transin, is a proteoglycanase closely related to collagenase (MMP1) with a wide range of substrate specificities. The complete primary structure for human MMP-3, which has 477 residues including a 17-residue signal peptide. MMP-3 and collagenase are 54% identical in sequence, suggesting a common origin for the evolution of the two proteinases. MMP-3 and collagenase expression are coordinately modulated in synovial fibroblast cultures.1 MMP-3 is a secreted metalloprotease produced predominantly by connective tissue cells. Together with other metalloproteases, it can synergistically degrade the major components of the extracellular matrix. It is capable of degrading proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen. MMP-3 genotype may be an important determinant of vascular remodeling and age-related arterial stiffening, with the heterozygote having the optimal balance between matrix accumulation and deposition.2 The standard product used in this kit is recombinant human MMP-3, consisting of 460 amino acids with the molecular mass of 52kD. The detected MMP-3 includes zymogen and active enzyme.

    限制

    仅限研究用
  • 储存条件

    4 °C/-20 °C
  • 抗原 See all MMP3 ELISA试剂盒

    MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))

    别名

    MMP-3

    基因ID

    4314

    UniProt

    P08254
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