MMP3 ELISA 试剂盒
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Quick Overview for MMP3 ELISA 试剂盒 (ABIN2042605)
抗原
See all MMP3 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 6 pg/mL
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交叉反应 （详细）
- There is cross-reactivates with MMP-10 approximately 2 % , and no detectable cross-reactivity with other MMPs.
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灵敏度
- < 10 pg/mL
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组件
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96-well plate pre-coated with anti-MMP-3, Human
Lyophilized recombinant MMP-3, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA Kit
MMP3 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: Cow Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: 人 AA 18-477 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: 犬 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: 大鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: 兔 Colorimetric Sandwich ELISA 2.19 ng/mL - 140 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: Pig Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Sandwich ELISA quantitative detection kit for Human MMP-3 in cell culture supernates, serum and plasma (heparin).Matrix metalloproteinase-3 (MMP-3) also called stromelysin or transin, is a proteoglycanase closely related to collagenase (MMP1) with a wide range of substrate specificities. The complete primary structure for human MMP-3, which has 477 residues including a 17-residue signal peptide. MMP-3 and collagenase are 54% identical in sequence, suggesting a common origin for the evolution of the two proteinases. MMP-3 and collagenase expression are coordinately modulated in synovial fibroblast cultures.1 MMP-3 is a secreted metalloprotease produced predominantly by connective tissue cells. Together with other metalloproteases, it can synergistically degrade the major components of the extracellular matrix. It is capable of degrading proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen. MMP-3 genotype may be an important determinant of vascular remodeling and age-related arterial stiffening, with the heterozygote having the optimal balance between matrix accumulation and deposition.2 The standard product used in this kit is recombinant human MMP-3, consisting of 460 amino acids with the molecular mass of 52kD. The detected MMP-3 includes zymogen and active enzyme.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))
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别名
- MMP-3
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基因ID
- 4314
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UniProt
- P08254
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