MMP2 ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for MMP2 ELISA 试剂盒 (ABIN2042603)
抗原
See all MMP2 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
-
-
最低检测浓度
- 6 pg/mL
-
交叉反应 (详细)
- There is no detectable cross-reactivity with other relevant proteins.
-
灵敏度
- < 10 pg/mL
-
组件
-
96-well plate pre-coated with anti-MMP-2, Mouse
Lyophilized recombinant MMP-2, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
-
-
-
-
板类型
- Pre-coated
-
实验流程
- Sandwich ELISA quantitative detection kit for Mouse MMP-2 in cell culture supernates, serum and plasma (heparin).Type IV collagenase, 72kD, is officially designated matrix metalloproteinase-2 (MMP2). It is also known as gelatinase, 72kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown.1 Matrix metalloproteinases (MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide (cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability.2 The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization.3 The standard product used in this kit is recombinant mouse MMP-2, consisting of 662 amino acids with the molecular mass of 72kD. The detected MMP-2 includes zymogen and active enzyme.
-
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C/-20 °C
-
-
- MMP2 (Matrix Metalloproteinase 2 (MMP2))
-
别名
- MMP-2
-
基因ID
- 17390
-
UniProt
- P33434
-
途径
- Activation of Innate immune Response
抗原 See all MMP2 ELISA试剂盒
-