MIF ELISA 试剂盒
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Quick Overview for MIF ELISA 试剂盒 (ABIN2042595)
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检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 6 pg/mL
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 20 pg/mL
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组件
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96-well plate pre-coated with anti-MIF, Human
Lyophilized recombinant MIF, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA Kit
MIF 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 兔 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitWide Range MIF 适用: 大鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 人 AA 2-115 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Serum
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 猴 AA 2-115 Colorimetric Sandwich ELISA
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 人 Colorimetric Sandwich ELISA 32-2000 pg/mL Cell Lysate, Plasma, Serum
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 人 Colorimetric Sandwich ELISA 781 pg/mL - 50000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Sandwich ELISA quantitative detection kit for Human MIF in cell culture supernates, serum and plasma (heparin, EDTA) and human milk.Macrophage migration inhibitory factor (MIF) is a protein which in humans is encoded by the MIF gene.1, 2 This gene is located to human chromosome 22q11.2.3 It is remarkably small, it has 3 exons separated by introns of only 189 and 95 bp, and covers less than 1 kb.4 This gene encodes a lymphokine that may be involved in cell-mediated immunity, immunoregulation, and inflammation.5 MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein might form a complex in the cytosol near the peripheral plasma membrane, which may possibly indicate a role in integrin signaling pathways. MIF also plays a central role in the toxic response to endotoxemia and possibly septic shock.Macrophage migration inhibitory factor has been reported to interact with COP9 constitutive photomorphogenic homolog subunit 5, CD74, BNIPL, and CXCR4. The standard product used in this kit is recombinant human MIF, consisting of 115 amino acids with the molecular mass of 12.5kD.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))
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别名
- MIF
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基因ID
- 4282
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UniProt
- I4AY87
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途径
- Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
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