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IGFBP3 ELISA 试剂盒

This 人 IGFBP3 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 IGFBP3.
产品编号 ABIN2042506
发货至: 中国

Quick Overview for IGFBP3 ELISA 试剂盒 (ABIN2042506)

抗原

See all IGFBP3 ELISA试剂盒
IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

156.2-10000 pg/mL

应用范围

ELISA
  • 最低检测浓度

    156.2 pg/mL

    交叉反应 (详细)

    There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    96-well plate pre-coated with anti-IGFBP-3, Human
    Lyophilized recombinant IGFBP-3, Human standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • 板类型

    Pre-coated

    实验流程

    Sandwich ELISA quantitative detection kit for Human IGFBP-3 in cell culture supernates, serum and plasma (heparin, EDTA).Insulin-like growth factor (IGF)-binding protein-3 (IGFBP-3) is a major determinant of circulating levels of the IGFs and is clinically useful for the evaluation of GH deficiency and for predicting the response to GH treatment. The circulating level of IGFBP-3 is inversely related to the risk of several common cancers, and that antiproliferative agents such as antiestrogens and retinoids act in part by up-regulating IGFBP-3 gene (IGFBP3) expression.1 Insulin-like growth factor-binding protein (IGFBP)-3, well characterized as the carrier of insulin-like growth factor (IGF), has been reported to have intrinsic bioactivity that is independent of IGF binding. IGFBP-3 has an IGF-independent, antiproliferative effect in undifferentiated and early differentiated but not in terminally differentiated chondrocytes.2 IGFBP-3 possesses both growth-inhibitory and potentiating effects on cells that are independent of IGF action and are mediated through specific. IGFBP-3 binding proteins/receptors locate at the cell membrane, cytosol, or nuclear compartments and in the extrIGFBP-3llular matrix.3 IGFBP3 is also located on chromosome 7.4 The standard product used in this kit is recombinant human IGFBP-3, consisting of 265 amino acids with the molecular mass of 29kD. As a result of glycosylation, the molecular mass is 41kD.

    限制

    仅限研究用
  • 储存条件

    4 °C/-20 °C
  • 抗原 See all IGFBP3 ELISA试剂盒

    IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

    别名

    IGFBP-3

    基因ID

    3486

    UniProt

    B3KPF0

    途径

    Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding
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