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FGF21 ELISA 试剂盒

This 人 FGF21 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 FGF21.
产品编号 ABIN2042462
发货至: 中国

Quick Overview for FGF21 ELISA 试剂盒 (ABIN2042462)

抗原

See all FGF21 ELISA试剂盒
FGF21 (Fibroblast Growth Factor 21 (FGF21))

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

31.2-2000 pg/mL

应用范围

ELISA
  • 最低检测浓度

    31.2 pg/mL

    交叉反应 (详细)

    There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    96-well plate pre-coated with anti-FGF21, Human
    Lyophilized recombinant FGF21, Human standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • 板类型

    Pre-coated

    实验流程

    Sandwich ELISA quantitative detection kit for Human FGF21 in cell culture supernates, serum and plasma (heparin, EDTA).Fibroblast growth factor 21 is a protein that in humans is encoded by the FGF21 gene. By genomic sequence analysis, Nishimura et al. (2000) identified the FGF21 gene within the 5-prime flanking region of the FUT1 gene on chromosome 19. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 injection in ob/ob mice results in an increase in Glut1 in adipose tissue. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents.

    限制

    仅限研究用
  • 储存条件

    4 °C/-20 °C
  • 抗原 See all FGF21 ELISA试剂盒

    FGF21 (Fibroblast Growth Factor 21 (FGF21))

    别名

    FGF21

    基因ID

    26291

    UniProt

    Q9NSA1

    途径

    RTK signaling
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