PI3 ELISA 试剂盒
Quick Overview for PI3 ELISA 试剂盒 (ABIN2042447)
抗原
See all PI3 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 31.2 pg/mL
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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96-well plate pre-coated with anti-Elafin/PI3, Human
Lyophilized recombinant Elafin/PI3, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Peptidase Inhibitor 3, Skin-Derived (PI3) ELISA Kit
PI3 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Saliva, Serum, Tissue Homogenate, Urine
Peptidase Inhibitor 3, Skin-Derived (PI3) ELISA KitPI3 适用: 人 AA 23-117 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Peptidase Inhibitor 3, Skin-Derived (PI3) ELISA KitPI3 适用: 人 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Plasma, Serum
Peptidase Inhibitor 3, Skin-Derived (PI3) ELISA KitPI3 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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板类型
- Pre-coated
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实验流程
- Sandwich ELISA quantitative detection kit for Human Elafin/PI3 in cell culture supernates, serum and plasma (heparin, EDTA).Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoproteinase (SKALP) is a protein that in humans is encoded by the PI3 gene. It is mapped to 20q13.12. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. This gene is the neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))
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别名
- Elafin/PI3
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基因ID
- 5266
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UniProt
- P19957
抗原 See all PI3 ELISA试剂盒
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