PI3 ELISA 试剂盒
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- 抗原 See all PI3 ELISA试剂盒
- PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))
- 抗原表位
- AA 23-117
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Elafin/PI3
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: A23-Q117 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: A23-Q117 - Featured
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Peptidase Inhibitor 3, Skin-Derived (PI3) ELISA Kit
PI3 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Saliva, Serum, Tissue Homogenate, Urine
Peptidase Inhibitor 3, Skin-Derived (PI3) ELISA KitPI3 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- human Elafin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Elafin has been precoated onto 96-well plates. Standards(NSO, A23-Q117) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Elafin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Elafin amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Elafin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Elafin standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 225, Standard deviation: 12.6, CV(%): 5.6
- Sample 2: n=16, Mean(pg/ml): 657, Standard deviation: 32.2, CV(%): 4.9
- Sample 3: n=16, Mean(pg/ml): 1334, Standard deviation: 57.36, CV(%): 4.3,
- Sample 1: n=24, Mean(pg/ml): 230, Standard deviation: 17.02, CV(%): 7.4
- Sample 2: n=24, Mean(pg/ml): 645, Standard deviation: 39.99, CV(%): 6.2
- Sample 3: n=24, Mean(pg/ml): 1742, Standard deviation: 113.23, CV(%): 6.5
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all PI3 ELISA试剂盒
- PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))
- 别名
- PI3 (PI3 产品)
- 别名
- ESI ELISA Kit, SKALP ELISA Kit, WAP3 ELISA Kit, WFDC14 ELISA Kit, cementoin ELISA Kit, trappin-2 ELISA Kit, TRAPPIN-2 ELISA Kit, elafin ELISA Kit, peptidase inhibitor 3 ELISA Kit, peptidase inhibitor 3, skin-derived (SKALP) ELISA Kit, PI3 ELISA Kit
- 背景
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Protein Function: Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.
Background: Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoproteinase(SKALP) is a protein that in humans is encoded by the PI3 gene. It is mapped to 20q13.12. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core(WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. This gene is the neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.
Synonyms: Elafin,Elastase-specific inhibitor,ESI,Peptidase inhibitor 3,PI-3,Protease inhibitor WAP3,Skin-derived antileukoproteinase,SKALP,WAP four-disulfide core domain protein 14,PI3,WAP3, WFDC14,
Full Gene Name: Elafin
Cellular Localisation: Secreted. - 基因ID
- 5266
- UniProt
- P19957
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