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CD14 ELISA 试剂盒

This 小鼠 CD14 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 CD14.
产品编号 ABIN2042389
发货至: 中国

Quick Overview for CD14 ELISA 试剂盒 (ABIN2042389)

抗原

See all CD14 ELISA试剂盒
CD14

适用

  • 14
  • 7
  • 6
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

6-10000 pg/mL

应用范围

ELISA
  • 最低检测浓度

    6 pg/mL

    交叉反应 (详细)

    There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    96-well plate pre-coated with anti-CD14, Mouse
    Lyophilized recombinant CD14, Mouse standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • 板类型

    Pre-coated

    实验流程

    Sandwich ELISA quantitative detection kit for Mouse CD14 in cell culture supernates, serum and plasma (heparin, EDTA).CD14, Cluster of differentiation 14, ingle-copy gene encoding 2 protein forms a 50- to 55kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.

    限制

    仅限研究用
  • 储存条件

    4 °C/-20 °C
  • 抗原 See all CD14 ELISA试剂盒

    CD14

    别名

    CD14

    基因ID

    12475

    UniProt

    P10810

    途径

    TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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