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CD80 ELISA 试剂盒

The 小鼠 CD80 ELISA Kit (ABIN2042352) is a Colorimetric ELISA Kit designed to quantify 小鼠 CD80.
产品编号 ABIN2042352
发货至: 中国
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Quick Overview for CD80 ELISA 试剂盒 (ABIN2042352)

抗原

See all CD80 ELISA试剂盒
CD80

适用

  • 10
  • 8
  • 6
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

62.5-4000 pg/mL

应用范围

ELISA
  • 最低检测浓度

    62.5 pg/mL

    交叉反应 (详细)

    There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    96-well plate pre-coated with anti-B7-1/CD80, Mouse
    Lyophilized recombinant B7-1/CD80, Mouse standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • 板类型

    Pre-coated

    实验流程

    Sandwich ELISA quantitative detection kit for Mouse B7-1/CD80 in cell culture supernates and serum.The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7.1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain[1].The CD80 and CD86 (601020) genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types.[2] stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4. The standard product used in this kit is recombinant B7-1 D37-K245 which is composed of two single chains acids with the dipolymer.

    限制

    仅限研究用
  • 储存条件

    4 °C/-20 °C
  • 抗原 See all CD80 ELISA试剂盒

    CD80

    别名

    B7-1/CD80

    基因ID

    12519

    UniProt

    Q00609

    途径

    TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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