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Angiopoietin 2 ELISA 试剂盒

This 人 Angiopoietin 2 ELISA Kit specifically detects Angiopoietin 2 .
产品编号 ABIN2042346
发货至: 中国
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Quick Overview for Angiopoietin 2 ELISA 试剂盒 (ABIN2042346)

抗原

See all Angiopoietin 2 (ANGPT2) ELISA试剂盒
Angiopoietin 2 (ANGPT2)

适用

  • 10
  • 7
  • 5
  • 4
  • 4
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

6-10000 pg/mL

应用范围

ELISA
  • 最低检测浓度

    6 pg/mL

    交叉反应 (详细)

    There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    96-well plate pre-coated with anti-Angiopoietin-2, Human
    Lyophilized recombinant Angiopoietin-2, Human standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • 板类型

    Pre-coated

    实验流程

    Sandwich ELISA quantitative detection kit for Human Angiopoietin-2 in cell culture supernates, serum, plasma (heparin, EDTA), saliva and human milk.Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2. Angiopoietin 2 is only 60% homologous with Angiopoietin 1. Angiopoietin-2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation. Angiopoietin 1 binding to Tie 2 causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodelling dorsal aorta and major aortic branches, ovary, placenta and uterus.

    限制

    仅限研究用
  • 储存条件

    4 °C/-20 °C
  • 抗原 See all Angiopoietin 2 (ANGPT2) ELISA试剂盒

    Angiopoietin 2 (ANGPT2)

    别名

    Angiopoietin-2

    基因ID

    285

    UniProt

    O15123

    途径

    RTK signaling
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