CD70 ELISA 试剂盒
Quick Overview for CD70 ELISA 试剂盒 (ABIN1889449)
抗原
See all CD70 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 31.2 pg/mL
-
原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD70/TNFSF7/cd27L
-
品牌
- PicoKine™
-
Analytical Method
- Quantitative
-
特异性
-
Expression system for standard: NSO
Immunogen sequence: S45-P195 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
-
灵敏度
- <10pg/mL
-
试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
-
免疫原
-
Expression system for standard: NSO
Immunogen sequence: S45-P195
-
-
CD70 Molecule (CD70) ELISA Kit
CD70 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
CD70 Molecule (CD70) ELISA KitCD70 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
-
-
应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
-
说明
-
Tissue Specificity: Very low level of expression. Detected in splenocytes and thymocytes.
-
板类型
- Pre-coated
-
实验流程
- mouse CD70 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD70 has been precoated onto 96-well plates. Standards(NSO, S45-P195) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD70 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD70 amount of sample captured in plate.
-
实验流程
-
Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse CD70 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD70 standard solution and each sample be measured in duplicate.
-
实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 94, Standard deviation: 3.57, CV(%): 3.8
- Sample 2: n=16, Mean(pg/ml): 511, Standard deviation: 26.06, CV(%): 5.1
- Sample 3: n=16, Mean(pg/ml): 1032, Standard deviation: 44.38, CV(%): 4.3,
- Sample 1: n=24, Mean(pg/ml): 148, Standard deviation: 6.66, CV(%): 4.5
- Sample 2: n=24, Mean(pg/ml): 525, Standard deviation: 32.55, CV(%): 6.2
- Sample 3: n=24, Mean(pg/ml): 1385, Standard deviation: 77.56, CV(%): 5.6
-
限制
- 仅限研究用
-
-
-
注意事项
- Avoid multiple freeze-thaw cycles.
-
储存条件
- -20 °C,4 °C
-
储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
-
有效期
- 12 months
-
-
- CD70 (CD70 Molecule (CD70))
-
别名
- CD70
-
背景
-
Protein Function: Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells. .
Background: CD70(Cluster of Differentiation 70), also known as TNFSF7 or CD27L, is a ligand for CD27. This gene is mapped to 19p13.3. CD70 is a surface antigen found on activated, but not resting, T and B lymphocytes. It has been found that CD70 induced proliferation of costimulated T cells and enhanced generation of cytolytic T cells. CD70 is homologous to the ligands of the TNF receptor family, including TNF-alpha, TNF-beta, and the CD40 ligand, showing 19 to 24 % amino acid sequence identity in the extracellular region.
Synonyms: CD70 antigen,CD27 ligand,CD27-L,Tumor necrosis factor ligand superfamily member 7,CD70,Cd70,Cd27l, Cd27lg, Tnfsf7,
Full Gene Name: CD70 antigen
Cellular Localisation: Membrane, Single-pass type II membrane protein. -
基因ID
- 21948
-
UniProt
- O55237
-
途径
- Cancer Immune Checkpoints
抗原 See all CD70 ELISA试剂盒
-
