电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

Betacellulin ELISA 试剂盒

This anti-Betacellulin antibody (ABIN1889437) is a antibody detecting Betacellulin in ELISA. Suitable for Rat.
产品编号 ABIN1889437
发货至: 中国

Quick Overview for Betacellulin ELISA 试剂盒 (ABIN1889437)

抗原

See all Betacellulin (BTC) ELISA试剂盒
Betacellulin (BTC)

抗原表位

AA 32-111

适用

  • 5
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
大鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

7.8-500 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
  • 最低检测浓度

    7.8 pg/mL

    原理

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat BTC

    品牌

    PicoKine™

    Analytical Method

    Quantitative

    特异性

    Expression system for standard: E.coli
    Immunogen sequence: D32-Y111

    交叉反应 (详细)

    There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    <10pg/mL

    试剂未包括

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    免疫原

    Expression system for standard: E.coli
    Immunogen sequence: D32-Y111
  • 应用备注

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    板类型

    Pre-coated

    实验流程

    rat BTC ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BTC has been precoated onto 96-well plates. Standards(E.coli, D32-Y111) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BTC is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat BTC amount of sample captured in plate.

    实验流程

    Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL rat BTC standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum, plasma(heparin, EDTA), tissue homogenates or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat BTC standard solution and each sample be measured in duplicate.

    实验精密度

    • Sample 1: n=16, Mean(pg/ml): 95, Standard deviation: 3.61, CV(%): 3.8
    • Sample 2: n=16, Mean(pg/ml): 178, Standard deviation: 7.3, CV(%): 4.1
    • Sample 3: n=16, Mean(pg/ml): 362, Standard deviation: 16.29, CV(%): 4.5,
    • Sample 1: n=24, Mean(pg/ml): 105, Standard deviation: 4.62, CV(%): 4.4
    • Sample 2: n=24, Mean(pg/ml): 241, Standard deviation: 12.53, CV(%): 5.2
    • Sample 3: n=24, Mean(pg/ml): 413, Standard deviation: 23.13, CV(%): 5.6

    限制

    仅限研究用
  • 注意事项

    Avoid multiple freeze-thaw cycles.

    储存条件

    -20 °C,4 °C

    储存方法

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    有效期

    12 months
  • 抗原 See all Betacellulin (BTC) ELISA试剂盒

    Betacellulin (BTC)

    别名

    BTC

    背景

    Background: Betacellulin, also known as BTC is a protein that in humans is encoded by the BTC gene. This gene is mapped to 4q13.3. The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. It is the growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. This protein is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.

    Synonyms: Betacellulin ,Protein Btc ,Btc ,BTC ,

    Full Gene Name: betacellulin

    基因ID

    64022

    UniProt

    Q9JJM4

    途径

    RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
You are here:
Chat with us!