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Angiopoietin 1 ELISA 试剂盒

This 小鼠 Angiopoietin 1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 Angiopoietin 1. This ELISA Kit has been cited in 2+ publications.
产品编号 ABIN1889436
发货至: 中国

Quick Overview for Angiopoietin 1 ELISA 试剂盒 (ABIN1889436)

抗原

See all Angiopoietin 1 (ANGPT1) ELISA试剂盒
Angiopoietin 1 (ANGPT1)

抗原表位

AA 16-497

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

156-10.000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
  • 最低检测浓度

    156 pg/mL

    原理

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Angiopoietin-1

    品牌

    PicoKine™

    Analytical Method

    Quantitative

    特异性

    Expression system for standard: NSO
    Immunogen sequence: H16-F497

    交叉反应 (详细)

    There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    <10pg/mL

    试剂未包括

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    免疫原

    Expression system for standard: NSO
    Immunogen sequence: H16-F497
  • 应用备注

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    板类型

    Pre-coated

    实验流程

    mouse Angiopoietin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Angiopoietin-1 has been precoated onto 96-well plates. Standards(NSO, H16-F497) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Angiopoietin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Angiopoietin-1 amount of sample captured in plate.

    实验流程

    Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Angiopoietin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Angiopoietin-1 standard solution and each sample be measured in duplicate.

    实验精密度

    • Sample 1: n=16, Mean(ng/ml): 1.28, Standard deviation: 0.055, CV(%): 4.3
    • Sample 2: n=16, Mean(ng/ml): 3.54, Standard deviation: 0.131, CV(%): 3.7
    • Sample 3: n=16, Mean(ng/ml): 6.85, Standard deviation: 0.301, CV(%): 4.4,
    • Sample 1: n=24, Mean(ng/ml): 1.59, Standard deviation: 0.086, CV(%): 5.4
    • Sample 2: n=24, Mean(ng/ml): 3.83, Standard deviation: 0.181, CV(%): 4.7
    • Sample 3: n=24, Mean(ng/ml): 6.97, Standard deviation: 0.369, CV(%): 5.3

    限制

    仅限研究用
  • 注意事项

    Avoid multiple freeze-thaw cycles.

    储存条件

    -20 °C,4 °C

    储存方法

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    有效期

    12 months
  • Schlosser, Taha, Deng, McIntyre, Mei, Stewart: "High circulating angiopoietin-2 levels exacerbate pulmonary inflammation but not vascular leak or mortality in endotoxin-induced lung injury in mice." in: Thorax, Vol. 73, Issue 3, pp. 248-261, (2018) (PubMed).

    Burnett, Gomez, De Leon, Ariaans, Progias, Kammerer, Velasco, Marron, Hellewell, Ridger: "Angiopoietin-1 enhances neutrophil chemotaxis in vitro and migration in vivo through interaction with CD18 and release of CCL4." in: Scientific reports, Vol. 7, Issue 1, pp. 2332, (2017) (PubMed).

  • 抗原 See all Angiopoietin 1 (ANGPT1) ELISA试剂盒

    Angiopoietin 1 (ANGPT1)

    别名

    ANGPT1

    背景

    Protein Function: Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme (By similarity). .

    Background: Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.

    Synonyms: Angiopoietin-1,ANG-1,Angpt1,Agpt,

    Full Gene Name: Angiopoietin-1

    Cellular Localisation: Secreted.

    基因ID

    11600

    UniProt

    O08538

    途径

    RTK signaling, Glycosaminoglycan Metabolic Process
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