Angiopoietin 1 ELISA 试剂盒
(1 reference)Quick Overview for Angiopoietin 1 ELISA 试剂盒 (ABIN1889435)
抗原
See all Angiopoietin 1 (ANGPT1) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat Angiopoietin-1
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: H16-F498 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: H16-F498
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Angiopoietin 1 (ANGPT1) ELISA Kit
ANGPT1 适用: 人 Colorimetric Sandwich ELISA 1.25-80 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Angiopoietin 1 (ANGPT1) ELISA KitANGPT1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiopoietin 1 (ANGPT1) ELISA KitANGPT1 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Angiopoietin 1 (ANGPT1) ELISA KitANGPT1 适用: Pig Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Angiopoietin 1 (ANGPT1) ELISA KitSmall Sample ANGPT1 适用: 人 Colorimetric Sandwich ELISA 0.24 ng/mL - 15 ng/mL Plasma, Serum, Tissue Homogenate
Angiopoietin 1 (ANGPT1) ELISA KitANGPT1 适用: 犬 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Angiopoietin 1 (ANGPT1) ELISA KitANGPT1 适用: Cow Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Angiopoietin 1 (ANGPT1) ELISA KitANGPT1 适用: 小鼠 AA 16-497 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Angiopoietin 1 (ANGPT1) ELISA KitANGPT1 适用: 人 AA 16-498 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum
Angiopoietin 1 (ANGPT1) ELISA KitANGPT1 适用: 人 Colorimetric Sandwich ELISA 157-10000 pg/mL Cell Lysate, Plasma, Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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板类型
- Pre-coated
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实验流程
- rat Angiopoietin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Angiopoietin-1 has been precoated onto 96-well plates. Standards(NSO, H16-F498) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Angiopoietin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Angiopoietin-1 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL rat Angiopoietin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat Angiopoietin-1 standard solution and each sample is measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.31, Standard deviation: 0.046, CV(%): 3.5
- Sample 2: n=16, Mean(ng/ml): 3.64, Standard deviation: 0.138, CV(%): 3.8
- Sample 3: n=16, Mean(ng/ml): 6.95, Standard deviation: 0.292, CV(%): 4.2,
- Sample 1: n=24, Mean(ng/ml): 1.42, Standard deviation: 0.058, CV(%): 4.1
- Sample 2: n=24, Mean(ng/ml): 3.75, Standard deviation: 0.173, CV(%): 4.6
- Sample 3: n=24, Mean(ng/ml): 7.03, Standard deviation: 0.401, CV(%): 5.7
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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: "Biocompatibility of porcine small intestinal submucosa and rat endothelial progenitor cells in vitro." in: International journal of clinical and experimental pathology, Vol. 8, Issue 2, pp. 1282-91, (2016) (PubMed).
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: "Biocompatibility of porcine small intestinal submucosa and rat endothelial progenitor cells in vitro." in: International journal of clinical and experimental pathology, Vol. 8, Issue 2, pp. 1282-91, (2016) (PubMed).
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- Angiopoietin 1 (ANGPT1)
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别名
- ANGPT1
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背景
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Protein Function: Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.
Background: Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.
Synonyms: Angiopoietin-1,ANG-1,Angpt1,
Full Gene Name: Angiopoietin-1
Cellular Localisation: Secreted. -
基因ID
- 89807
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UniProt
- O35460
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途径
- RTK signaling, Glycosaminoglycan Metabolic Process
抗原 See all Angiopoietin 1 (ANGPT1) ELISA试剂盒
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