TNFRSF11A ELISA 试剂盒
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Quick Overview for TNFRSF11A ELISA 试剂盒 (ABIN1889423)
抗原
See all TNFRSF11A ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat RANK
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: Q30-P213 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: Q30-P213
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Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A) ELISA Kit
TNFRSF11A 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A) ELISA KitTNFRSF11A 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A) ELISA KitTNFRSF11A 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A) ELISA KitTNFRSF11A 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A) ELISA KitTNFRSF11A 适用: 人 AA 29-213 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A) ELISA KitTNFRSF11A 适用: 小鼠 AA 30-213 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A) ELISA KitTNFRSF11A 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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板类型
- Pre-coated
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实验流程
- rat RANK ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for RANK has been precoated onto 96-well plates. Standards(NSO, Q30-P213) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for RANK is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat RANK amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL rat RANK standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat RANK standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 880, Standard deviation: 36.96, CV(%): 4.2
- Sample 2: n=16, Mean(pg/ml): 1954, Standard deviation: 107.5, CV(%): 5.5
- Sample 3: n=16, Mean(pg/ml): 2755, Standard deviation: 159.8, CV(%): 5.8,
- Sample 1: n=24, Mean(pg/ml): 950, Standard deviation: 51.3, CV(%): 5.4
- Sample 2: n=24, Mean(pg/ml): 2160, Standard deviation: 138.2, CV(%): 6.4
- Sample 3: n=24, Mean(pg/ml): 3052, Standard deviation: 228.9, CV(%): 7.5
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- TNFRSF11A (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A))
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别名
- TNFRSF11A
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背景
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Background: Receptor Activator of Nuclear Factor i° B (RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is a recently described TNF receptor family member, and its ligand, RANKL, promote survival of dendritic cells and differentiation of osteoclasts. RANK contains 383 amino acids in its intracellular domain(residues 234-616), which contain three putative TRAF-binding domains(termed I, II, and III). RANK interacts with various TRAFs through distinct motifs and activates NF-kappaB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kappaB activation, whereas RANK most likely activates JNK through a TRAF2-interacting region in RANK. The standard in this kit is recombinant human RANK with the sequence of Q29-G213 aa. It is a dipolymer which compose of two chains, and the molecular weight of each is 48kda.
Synonyms: Protein Tnfrsf11a ,Tnfrsf11a ,
Full Gene Name: tumor necrosis factor receptor superfamily, member 11a, NFKB activator
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基因ID
- 498206
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UniProt
- F1M8Z6
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途径
- NF-kappaB Signaling
抗原 See all TNFRSF11A ELISA试剂盒
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