LBP ELISA 试剂盒
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- 抗原 See all LBP ELISA试剂盒
- LBP (Lipopolysaccharide Binding Protein (LBP))
- 抗原表位
- AA 25-481
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 1.56-100 ng/mL
- 最低检测浓度
- 1.56 ng/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse LBP
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: G25-V481 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <50pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: G25-V481 - Featured
- Discover our best selling LBP ELISA Kit
- Top Product
- Discover our top product LBP ELISA Kit
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Lipopolysaccharide Binding Protein (LBP) ELISA Kit
LBP 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Lipopolysaccharide Binding Protein (LBP) ELISA KitLBP 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Lipopolysaccharide Binding Protein (LBP) ELISA KitLBP 适用: 小鼠 Colorimetric Sandwich ELISA 3.13 ng/mL - 200 ng/mL Cell Culture Supernatant, Plasma, Serum
Lipopolysaccharide Binding Protein (LBP) ELISA KitLBP 适用: Pig Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Lipopolysaccharide Binding Protein (LBP) ELISA KitLBP 适用: Cow Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum
Lipopolysaccharide Binding Protein (LBP) ELISA KitLBP 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lipopolysaccharide Binding Protein (LBP) ELISA KitLBP 适用: 大鼠 Colorimetric Sandwich ELISA 1.88 ng/mL - 120 ng/mL Cell Culture Supernatant, Plasma, Serum
Lipopolysaccharide Binding Protein (LBP) ELISA KitLBP 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Lipopolysaccharide Binding Protein (LBP) ELISA KitLBP 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Lipopolysaccharide Binding Protein (LBP) ELISA KitLBP 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- mouse LBP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for LBP has been precoated onto 96-well plates. Standards(NSO, G25-V481) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LBP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse LBP amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL mouse LBP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse LBP standard solution and each sample is measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 5.61, Standard deviation: 0.32, CV(%): 5.7
- Sample 2: n=16, Mean(ng/ml): 33.8, Standard deviation: 1.55, CV(%): 4.6
- Sample 3: n=16, Mean(ng/ml): 68.2, Standard deviation: 3.41, CV(%): 5,
- Sample 1: n=24, Mean(ng/ml): 5.32, Standard deviation: 0.46, CV(%): 8.6
- Sample 2: n=24, Mean(ng/ml): 36.5, Standard deviation: 1.93, CV(%): 5.3
- Sample 3: n=24, Mean(ng/ml): 74.9, Standard deviation: 4.81, CV(%): 6.4
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "Reduction Impairs the Antibacterial Activity but Benefits the LPS Neutralization Ability of Human Enteric Defensin 5." in: Scientific reports, Vol. 6, pp. 22875, (2017) (PubMed).
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: "Reduction Impairs the Antibacterial Activity but Benefits the LPS Neutralization Ability of Human Enteric Defensin 5." in: Scientific reports, Vol. 6, pp. 22875, (2017) (PubMed).
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- 抗原 See all LBP ELISA试剂盒
- LBP (Lipopolysaccharide Binding Protein (LBP))
- 别名
- LBP (LBP 产品)
- 别名
- BPIFD2 ELISA Kit, Bpifd2 ELISA Kit, Ly88 ELISA Kit, LPSBP ELISA Kit, LBP ELISA Kit, lipopolysaccharide binding protein ELISA Kit, lipopolysaccharide-binding protein ELISA Kit, LBP ELISA Kit, Lbp ELISA Kit, LOC100472839 ELISA Kit
- 背景
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Protein Function: Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria, and acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide (By similarity). .
Background: Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene. This gene is mapped to 20q11.23. LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide(or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. It is present in the cerebrospinal fluid of patients with pneumococcal meningitis. The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. LBP is made in the liver during the acute phase of infections and is thought to function as a carrier for LPS and to help control LPS-dependent monocyte responses.
Synonyms: Lipopolysaccharide-binding protein,LBP,Lbp,
Full Gene Name: Lipopolysaccharide-binding protein
Cellular Localisation: Secreted. - 基因ID
- 16803
- UniProt
- Q61805
- 途径
- TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process
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