Osteoactivin ELISA 试剂盒
Quick Overview for Osteoactivin ELISA 试剂盒 (ABIN1889404)
抗原
See all Osteoactivin (GPNMB) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 156 pg/mL
-
原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human GPNMB/Osteoactivin
-
品牌
- PicoKine™
-
Analytical Method
- Quantitative
-
特异性
-
Expression system for standard: NSO
Immunogen sequence: A22-P486 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
-
灵敏度
- <10pg/mL
-
试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
-
免疫原
-
Expression system for standard: NSO
Immunogen sequence: A22-P486
-
-
Osteoactivin (GPNMB) ELISA Kit
GPNMB 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Osteoactivin (GPNMB) ELISA KitGPNMB 适用: 小鼠 AA 23-502 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Osteoactivin (GPNMB) ELISA KitGPNMB 适用: 小鼠 Colorimetric Sandwich ELISA 80-20.000 pg/mL Cell Culture Supernatant, Plasma, Serum
Osteoactivin (GPNMB) ELISA KitGPNMB 适用: 人 Colorimetric Sandwich ELISA 45-12.000 pg/mL Cell Culture Supernatant, Plasma, Serum
Osteoactivin (GPNMB) ELISA KitGPNMB 适用: 人 Colorimetric Sandwich ELISA Cell Culture Cells, Plasma, Serum
-
-
应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
-
说明
-
Sequence similarities: Belongs to the PMEL/NMB family.
Tissue Specificity: Up-regulated in various cancer cells, including in glioblastoma multiforme. Expressed in many melanoma cells, as well as in tissue macrophages, including liver Kuppfer cells and lung alveolar macrophages, in podocytes and in some cells of the ciliary body of the eye (at protein level). Hardly detectable in healthy brain. .
-
板类型
- Pre-coated
-
实验流程
- human GPNMB ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GPNMB has been precoated onto 96-well plates. Standards(NSO, A22-P486) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GPNMB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human GPNMB amount of sample captured in plate.
-
实验流程
-
Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human GPNMB standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human GPNMB standard solution and each sample be measured in duplicate.
-
实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 512, Standard deviation: 23.04, CV(%): 4.5
- Sample 2: n=16, Mean(pg/ml): 3635, Standard deviation: 170.8, CV(%): 4.7
- Sample 3: n=16, Mean(pg/ml): 5518, Standard deviation: 303.5, CV(%): 5.5,
- Sample 1: n=24, Mean(pg/ml): 621, Standard deviation: 42.85, CV(%): 5.9
- Sample 2: n=24, Mean(pg/ml): 3857, Standard deviation: 239.1, CV(%): 6.2
- Sample 3: n=24, Mean(pg/ml): 6026, Standard deviation: 391.7, CV(%): 6.5
-
限制
- 仅限研究用
-
-
-
注意事项
- Avoid multiple freeze-thaw cycles.
-
储存条件
- -20 °C,4 °C
-
储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
-
有效期
- 12 months
-
-
- Osteoactivin (GPNMB)
-
别名
- GPNMB
-
背景
-
Protein Function: Could be a melanogenic enzyme. .
Background: Transmembrane glycoprotein NMB is a protein that in humans is encoded by the GPNMB gene. In osteoblast progenitor cells, GPNMB works as a positive regulator of osteoblast differentiation during later stages of matrix maturation and mineralization that is mediated at least in part by BMP-2 in a SMAD1 dependent manner to promote osteoblast differentiation. GPNMB can enhance the repairing process in bone fracture, demonstrated by its high expression during chondrogenesis(soft callus) and osteogenesis(hard callus) compared to the intact femurs that is why Osteoactivin(OA) could be a novel therapeutic agent used to treat generalized osteoporosis or localized osteopenia during fracture repair by stimulating bone growth and regeneration. Similarly, GPNMB expression increases during osteoclast differentiation and it is functionally implicated in this process, possibly by promoting the fusion of osteoclast progenitor cells.
Synonyms: Transmembrane glycoprotein NMB,Transmembrane glycoprotein HGFIN,GPNMB,HGFIN, NMB,UNQ1725/PRO9925,
Full Gene Name: Transmembrane glycoprotein NMB
Cellular Localisation: Cell membrane, Single-pass type I membrane protein. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Detected at the cell surface in different types of cancers cells, including glioblastoma multiforme cells and most melanoma cell lines. -
基因ID
- 10457
-
UniProt
- Q14956
抗原 See all Osteoactivin (GPNMB) ELISA试剂盒
-
