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Renin ELISA 试剂盒

Name: Renin ELISA 试剂盒
SKU: ABIN1889403
Availability: OutOfStock

This 小鼠 Renin ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 Renin. This ELISA Kit has been cited in1 publication.

ELISA

1 image

(1 reference)

产品编号 ABIN1889403

发货至: 中国

Quick Overview for Renin ELISA 试剂盒 (ABIN1889403)

抗原

See all Renin (REN) ELISA试剂盒

Renin (REN)

抗原表位

AA 72-402

适用

小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

62.5-4000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Discover our top product REN ELISA Kit

最低检测浓度

62.5 pg/mL

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Renin-1

品牌

PicoKine™

Analytical Method

Quantitative

特异性

Expression system for standard: NSO
Immunogen sequence: S72-R402

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<10pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: NSO
Immunogen sequence: S72-R402
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应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

说明

Tissue Specificity: Kidney.

板类型

Pre-coated

实验流程

mouse Renin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Renin-1 has been precoated onto 96-well plates. Standards(NSO, S72-R402) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Renin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Renin-1 amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse Renin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Renin-1 standard solution and each sample be measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(pg/ml): 890, Standard deviation: 49.84, CV(%): 5.6
- Sample 2: n=16, Mean(pg/ml): 1137, Standard deviation: 48.9, CV(%): 4.3
- Sample 3: n=16, Mean(pg/ml): 2932, Standard deviation: 161.26, CV(%): 5.5,
- Sample 1: n=24, Mean(pg/ml): 927, Standard deviation: 67.7, CV(%): 7.3
- Sample 2: n=24, Mean(pg/ml): 2274, Standard deviation: 129.6, CV(%): 5.7
- Sample 3: n=24, Mean(pg/ml): 3045, Standard deviation: 194.88, CV(%): 6.4
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
Wang, Ren: "Preparation and characterization of polyclonal antibody against severe acute respiratory syndrome-associated coronavirus spike protein." in: Hybridoma (2005), Vol. 29, Issue 6, pp. 511-6, (2010) (PubMed).
抗原 See all Renin (REN) ELISA试剂盒

Renin (REN)

别名

Renin-1

背景

Protein Function: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
Background: Renin-1 is also known as Ren, Angiotensinogenase or Kidney rennin. It is a member of peptidase A1 family. Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. It associated to membranes via binding to ATP6AP2.
Synonyms: Renin-1,3.4.23.15,Angiotensinogenase,Kidney renin,Ren1,Ren, Ren-1,
Full Gene Name: Renin-1
Cellular Localisation: Secreted . Membrane . Associated to membranes via binding to ATP6AP2..

基因ID

19701

UniProt

P06281

途径

ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour