Relaxin 1 ELISA 试剂盒
Quick Overview for Relaxin 1 ELISA 试剂盒 (ABIN1889393)
抗原
See all Relaxin 1 (RLN1) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 78 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Relaxin 1
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: R23-R54(B)&E161-C185(A) -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: E.coli
Immunogen sequence: R23-R54(B)&E161-C185(A)
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Relaxin 1 (RLN1) ELISA Kit
RLN1 适用: 兔 Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Plasma, Serum
Relaxin 1 (RLN1) ELISA KitRLN1 适用: 大鼠 Colorimetric Sandwich ELISA 2.4 pg/mL - 150 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Relaxin 1 (RLN1) ELISA KitRLN1 适用: 人 Colorimetric Competition ELISA 3.2 pg/mL - 2000 pg/mL Plasma, Serum
Relaxin 1 (RLN1) ELISA KitRLN1 适用: 小鼠 Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Plasma, Serum
Relaxin 1 (RLN1) ELISA KitRLN1 适用: 大鼠 Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Plasma, Serum
Relaxin 1 (RLN1) ELISA KitRLN1 适用: 人 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate
Relaxin 1 (RLN1) ELISA KitRLN1 适用: 犬 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
Relaxin 1 (RLN1) ELISA KitRLN1 适用: Cow Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Plasma, Serum
Relaxin 1 (RLN1) ELISA KitRLN1 适用: Pig Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Relaxin 1 (RLN1) ELISA KitRLN1 适用: 人 AA 26-53 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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板类型
- Pre-coated
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实验流程
- mouse Relaxin 1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Relaxin 1 has been precoated onto 96-well plates. Standards(E.coli, R23-R54(B) & E161-C185(A)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Relaxin 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Relaxin 1 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL mouse Relaxin 1 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse Relaxin 1 standard solution and each sample is measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 688, Standard deviation: 45.41, CV(%): 6.6
- Sample 2: n=16, Mean(pg/ml): 1402, Standard deviation: 60.3, CV(%): 4.3
- Sample 3: n=16, Mean(pg/ml): 2366, Standard deviation: 130, CV(%): 5.5,
- Sample 1: n=24, Mean(pg/ml): 651, Standard deviation: 48.2, CV(%): 7.4
- Sample 2: n=24, Mean(pg/ml): 1583, Standard deviation: 95, CV(%): 6
- Sample 3: n=24, Mean(pg/ml): 2425, Standard deviation: 174.6, CV(%): 7.2
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- Relaxin 1 (RLN1)
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别名
- RLN1
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背景
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Protein Function: Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals.
Background: Relaxin 1 is a member of relaxin-like peptide family. Relaxin gene maps to mouse chromosome 19 near D19Mit23. Relaxin is a peptide hormone produced by the corpora lutea of ovaries during pregnancy in many mammalian species, including man. Relaxin widens the pubic bone and facilitates labor, it also softens the cervix(cervical ripening), and relaxes the uterine musculature. However, its significance may reach much further. Relaxin affects collagen metabolism, inhibiting collagen synthesis and enhancing its breakdown by increasing matrix metalloproteinases. It also enhances angiogenesis and is a potent renal vasodilator.
Synonyms: Prorelaxin 1,Relaxin B chain,Relaxin A chain,Rln1,Rln, Rlx,
Full Gene Name: Prorelaxin 1
Cellular Localisation: Secreted. -
基因ID
- 19773
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UniProt
- P47932
抗原 See all Relaxin 1 (RLN1) ELISA试剂盒
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