Relaxin 1 ELISA 试剂盒
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Quick Overview for Relaxin 1 ELISA 试剂盒 (ABIN2859323)
抗原
See all Relaxin 1 (RLN1) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 78 pg/mL
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原理
- Sandwich Human Relaxin 1 ELISA Kit to quantitate Human RLN1 in cell culture supernatants, serum and plasma (heparin).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: R162-Cys185 (A) & K26-S53 (B)Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Relaxin 1 (RLN1) ELISA Kit
RLN1 适用: 兔 Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Plasma, Serum
Relaxin 1 (RLN1) ELISA KitRLN1 适用: 人 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate
Relaxin 1 (RLN1) ELISA KitRLN1 适用: 犬 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
Relaxin 1 (RLN1) ELISA KitRLN1 适用: 大鼠 Colorimetric Sandwich ELISA 2.4 pg/mL - 150 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Relaxin 1 (RLN1) ELISA KitRLN1 适用: 小鼠 Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Plasma, Serum
Relaxin 1 (RLN1) ELISA KitRLN1 适用: 大鼠 Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Plasma, Serum
Relaxin 1 (RLN1) ELISA KitRLN1 适用: 人 Colorimetric Competition ELISA 3.2 pg/mL - 2000 pg/mL Plasma, Serum
Relaxin 1 (RLN1) ELISA KitRLN1 适用: Cow Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Plasma, Serum
Relaxin 1 (RLN1) ELISA KitRLN1 适用: Pig Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Relaxin 1 (RLN1) ELISA KitRLN1 适用: 兔 Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Plasma, Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 7.6%, 4.6%, 4.2%
Inter-Assay Precision (CV%): 7.9%, 5.4%, 4.6% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- Relaxin 1 (RLN1)
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别名
- RLN1
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背景
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Background: Relaxin 1 is a member of relaxin-like peptide family. Relaxin gene maps to human chromosome 19 near D19Mit23. Relaxin is a peptide hormone produced by the corpora lutea of ovaries during pregnancy in many mammalian species, including man. Relaxin widens the pubic bone and facilitates labor, it also softens the cervix (cervical ripening), and relaxes the uterine musculature. However, its significance may reach much further. Relaxin affects collagen metabolism, inhibiting collagen synthesis and enhancing its breakdown by increasing matrix metalloproteinases. It also enhances angiogenesis and is a potent renal vasodilator.
Gene Full Name: relaxin 1
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基因ID
- 6013
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UniProt
- P04808
抗原 See all Relaxin 1 (RLN1) ELISA试剂盒
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