WISP1 ELISA 试剂盒
Quick Overview for WISP1 ELISA 试剂盒 (ABIN1889366)
抗原
See all WISP1 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse WISP1/CCN4
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: T23-N367 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: T23-N367
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WNT1 Inducible Signaling Pathway Protein 1 (WISP1) ELISA Kit
WISP1 适用: 人 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
WNT1 Inducible Signaling Pathway Protein 1 (WISP1) ELISA KitWISP1 适用: 小鼠 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate
WNT1 Inducible Signaling Pathway Protein 1 (WISP1) ELISA KitWISP1 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
WNT1 Inducible Signaling Pathway Protein 1 (WISP1) ELISA KitWISP1 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
WNT1 Inducible Signaling Pathway Protein 1 (WISP1) ELISA KitWISP1 适用: 人 AA 23-367 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Belongs to the CCN family.
Tissue Specificity: Highly expressed in kidney and lung. Lower levels in heart, brain, spleen, liver, skeletal muscle and testis. Expressed in low metastatic melanoma cells.
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板类型
- Pre-coated
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实验流程
- mouse WISP1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for WISP1 has been precoated onto 96-well plates. Standards(NSO, T23-N367) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for WISP1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse WISP1 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse WISP1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse WISP1 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 125, Standard deviation: 6, CV(%): 4.8
- Sample 2: n=16, Mean(pg/ml): 670, Standard deviation: 26.13, CV(%): 3.9
- Sample 3: n=16, Mean(pg/ml): 1043, Standard deviation: 53.2, CV(%): 5.1,
- Sample 1: n=24, Mean(pg/ml): 183, Standard deviation: 14.1, CV(%): 7.7
- Sample 2: n=24, Mean(pg/ml): 762, Standard deviation: 51.82, CV(%): 6.8
- Sample 3: n=24, Mean(pg/ml): 1120, Standard deviation: 72.8, CV(%): 6.5
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
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别名
- WISP1
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背景
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Protein Function: Downstream regulator in the Wnt/Frizzled-signaling pathway (By similarity). Associated with cell survival. Adheres to skin and melanoma fibroblasts (By similarity). In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan (By similarity). Suppresses tumor growth in vivo. .
Background: WISP1(WNT1-Inducible Signaling Pathway Protein 1), also known as CCN4, is a matricellular protein that in humans is encoded by the WISP1 gene. WISP1 is induced by WNT1 and belongs to the CCN family, which includes connective tissue growth factor, cysteine-rich-61, and nephroblastoma overexpressed. By use of radiation hybrid mapping panels, the WISP1 was mapped to chromosome 8q24.1-q24.3, roughly 4 Mb distal to MYC. It found that 2 distinct systems demonstrated that WISP induction was associated with expression of WNT1. WISP1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors, and its RNA was overexpressed in 84 % of tumors examined compared with patient-matched normal mucosa.
Synonyms: WNT1-inducible-signaling pathway protein 1,WISP-1,CCN family member 4,ELM-1,Wisp1,Ccn4, Elm1,
Full Gene Name: WNT1-inducible-signaling pathway protein 1
Cellular Localisation: Secreted. -
基因ID
- 22402
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UniProt
- O54775
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途径
- WNT signaling, Growth Factor Binding
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