Betacellulin ELISA 试剂盒
Quick Overview for Betacellulin ELISA 试剂盒 (ABIN1889364)
抗原
See all Betacellulin (BTC) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 7.8 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Betacellulin/BTC
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: D32-Y111 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: E.coli
Immunogen sequence: D32-Y111
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Betacellulin (BTC) ELISA Kit
BTC 适用: 大鼠 Colorimetric Sandwich ELISA 9.375 pg/mL - 600 pg/mL Plasma, Serum, Tissue Homogenate
Betacellulin (BTC) ELISA KitBTC 适用: 小鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Betacellulin (BTC) ELISA KitBTC 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Betacellulin (BTC) ELISA KitBTC 适用: 人 Colorimetric Sandwich ELISA 30-18000 pg/mL Cell Culture Supernatant, Plasma, Serum
Betacellulin (BTC) ELISA KitBTC 适用: 人 AA 32-111 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Betacellulin (BTC) ELISA KitBTC 适用: 大鼠 AA 32-111 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Betacellulin (BTC) ELISA KitBTC 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Tissue Specificity: Found in several mouse tissues including kidney, uterus and liver, as well as in beta tumor cell line and MCF-7 cells. It is not detected in the brain.
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板类型
- Pre-coated
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实验流程
- mouse BTC ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for BTC has been precoated onto 96-well plates. Standards(E.coli, D32-Y111) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BTC is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse BTC amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL mouse BTC standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse BTC standard solution and each sample is measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 28.6, Standard deviation: 1.40, CV(%): 4.9
- Sample 2: n=16, Mean(pg/ml): 69.4, Standard deviation: 3.61, CV(%): 5.2
- Sample 3: n=16, Mean(pg/ml): 279, Standard deviation: 15.90, CV(%): 5.7,
- Sample 1: n=24, Mean(pg/ml): 29.3, Standard deviation: 1.70, CV(%): 5.8
- Sample 2: n=24, Mean(pg/ml): 69.8, Standard deviation: 4.40, CV(%): 6.3
- Sample 3: n=24, Mean(pg/ml): 285, Standard deviation: 23.09, CV(%): 8.1
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- Betacellulin (BTC)
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别名
- BTC
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背景
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Protein Function: Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. .
Background: Betacellulin, also known as BTC is a protein that in humans is encoded by the BTC gene. This gene is mapped to 4q13.3. The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. It is the growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. This protein is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.
Synonyms: Probetacellulin,Betacellulin,BTC,Btc,Bcn,
Full Gene Name: Probetacellulin
Cellular Localisation: Betacellulin: Secreted, extracellular space. -
基因ID
- 12223
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UniProt
- Q05928
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
抗原 See all Betacellulin (BTC) ELISA试剂盒
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