ACE2 ELISA 试剂盒
(1 reference)Quick Overview for ACE2 ELISA 试剂盒 (ABIN1889360)
抗原
See all ACE2 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse ACE2
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: CHO
Immunogen sequence: Q18-T740 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: CHO
Immunogen sequence: Q18-T740
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Angiotensin I Converting Enzyme 2 (ACE2) ELISA Kit
ACE2 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 人 Colorimetric Sandwich ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 人 Colorimetric Sandwich ELISA 0.39 ng/mL - 25 ng/mL Cell Culture Supernatant, Plasma, Serum
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 小鼠 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 人 Colorimetric Sandwich ELISA 0.025 ng/mL - 20 ng/mL Cell Culture Cells, Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Tissue Specificity: Expressed in heart, kidney and forebrain (at protein level). Ubiquitously expressed, with highest levels in ileum, kidney and lung. In lung, expressed on vascular endothelial and airway epithelial cells. .
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板类型
- Pre-coated
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实验流程
- mouse ACE2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for ACE2 has been precoated onto 96-well plates. Standards(CHO, Q18-T740) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ACE2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse ACE2 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse ACE2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse ACE2 standard solution and each sample is measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 417, Standard deviation: 20.85, CV(%): 5
- Sample 2: n=16, Mean(pg/ml): 1235, Standard deviation: 65.46, CV(%): 5.3
- Sample 3: n=16, Mean(pg/ml): 2279, Standard deviation: 127.62, CV(%): 5.6,
- Sample 1: n=24, Mean(pg/ml): 503, Standard deviation: 37.73, CV(%): 7.5
- Sample 2: n=24, Mean(pg/ml): 1327, Standard deviation: 102.18, CV(%): 7.7
- Sample 3: n=24, Mean(pg/ml): 2305, Standard deviation: 189.01, CV(%): 8.2
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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: "Aliskiren Improves Ischemia- and Oxygen Glucose Deprivation-Induced Cardiac Injury through Activation of Autophagy and AMP-Activated Protein Kinase." in: Frontiers in pharmacology, Vol. 8, pp. 819, (2017) (PubMed).
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: "Aliskiren Improves Ischemia- and Oxygen Glucose Deprivation-Induced Cardiac Injury through Activation of Autophagy and AMP-Activated Protein Kinase." in: Frontiers in pharmacology, Vol. 8, pp. 819, (2017) (PubMed).
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- ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))
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别名
- ACE2
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背景
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Protein Function: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin- 13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. May have a protective role in acute lung injury. .
Background: AngiotensinI-converting enzyme 2(ACE 2) is a protein belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. By sequence similarity to a sequence in GenBank, this gene is mapped to Xp22.2. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.
Synonyms: Angiotensin-converting enzyme 2,3.4.17.23,ACE-related carboxypeptidase,Processed angiotensin-converting enzyme 2,Ace2,
Full Gene Name: Angiotensin-converting enzyme 2
Cellular Localisation: Processed angiotensin-converting enzyme 2: Secreted. -
基因ID
- 70008
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UniProt
- Q8R0I0
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途径
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
抗原 See all ACE2 ELISA试剂盒
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