Sclerostin ELISA 试剂盒
(1 reference)Quick Overview for Sclerostin ELISA 试剂盒 (ABIN1889353)
抗原
See all Sclerostin (SOST) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Sclerostin/SOST
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: Q24-Y211 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: Q24-Y211
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Sclerostin (SOST) ELISA Kit
SOST 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Sclerostin (SOST) ELISA KitSOST 适用: 小鼠 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate
Sclerostin (SOST) ELISA KitSOST 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Sclerostin (SOST) ELISA KitSOST 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Sclerostin (SOST) ELISA KitSOST 适用: 人 AA 24-213 Colorimetric Sandwich ELISA
Sclerostin (SOST) ELISA KitSOST 适用: 大鼠 AA 29-213 Colorimetric Sandwich ELISA
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Belongs to the sclerostin family.
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板类型
- Pre-coated
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实验流程
- mouse Sclerostin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Sclerostin has been precoated onto 96-well plates. Standards(NSO, Q24-Y211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Sclerostin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Sclerostin amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse Sclerostin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well.. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Sclerostin standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 76, Standard deviation: 5.55, CV(%): 7.3
- Sample 2: n=16, Mean(pg/ml): 217, Standard deviation: 12.6, CV(%): 5.8
- Sample 3: n=16, Mean(pg/ml): 463, Standard deviation: 25.93, CV(%): 5.6,
- Sample 1: n=24, Mean(pg/ml): 88, Standard deviation: 7.13, CV(%): 8.1
- Sample 2: n=24, Mean(pg/ml): 225, Standard deviation: 14.4, CV(%): 6.4
- Sample 3: n=24, Mean(pg/ml): 478, Standard deviation: 28.68, CV(%): 6
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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: "Sclerostin Promotes Bone Remodeling in the Process of Tooth Movement." in: PLoS ONE, Vol. 12, Issue 1, pp. e0167312, (2017) (PubMed).
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: "Sclerostin Promotes Bone Remodeling in the Process of Tooth Movement." in: PLoS ONE, Vol. 12, Issue 1, pp. e0167312, (2017) (PubMed).
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- Sclerostin (SOST)
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别名
- SOST
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背景
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Protein Function: Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation. .
Background: Sclerostin is a protein that in humans is encoded by the SOST gene. Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like(CTCK) domain and sequence similarity to the DAN(differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein(BMP) antagonists. Sclerostin is produced by the osteocyte and has anti-anabolic effects on bone formation. The SOST gene maps to chromosome 17q12-q21
Synonyms: Sclerostin,Sost,
Full Gene Name: Sclerostin
Cellular Localisation: Secreted, extracellular space, extracellular matrix. -
基因ID
- 74499
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UniProt
- Q99P68
抗原 See all Sclerostin (SOST) ELISA试剂盒
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