FGF19 ELISA 试剂盒
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- 抗原 See all FGF19 ELISA试剂盒
- FGF19 (Fibroblast Growth Factor 19 (FGF19))
- 抗原表位
- AA 25-216
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6-1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human FGF19
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: L25-K216 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: L25-K216 - Featured
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- Discover our top product FGF19 ELISA Kit
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Fibroblast Growth Factor 19 (FGF19) ELISA Kit
FGF19 适用: 大鼠 Colorimetric Sandwich ELISA 9.38 pg/mL - 600 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 19 (FGF19) ELISA KitFGF19 适用: Pig Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 19 (FGF19) ELISA KitFGF19 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 19 (FGF19) ELISA KitFGF19 适用: 小鼠 Colorimetric Sandwich ELISA 9.4 pg/mL - 600 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Expressed in fetal brain, cartilage, retina, and adult gall bladder. .
- 板类型
- Pre-coated
- 实验流程
- human FGF19 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FGF19 has been precoated onto 96-well plates. Standards(E.coli, L25-K216) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF19 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FGF19 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human FGF19 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human FGF19 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 103, Standard deviation: 6.69, CV(%): 6.5
- Sample 2: n=16, Mean(pg/ml): 396, Standard deviation: 17, CV(%): 4.3
- Sample 3: n=16, Mean(pg/ml): 669, Standard deviation: 25.4, CV(%): 3.8,
- Sample 1: n=24, Mean(pg/ml): 152, Standard deviation: 10.94, CV(%): 7.2
- Sample 2: n=24, Mean(pg/ml): 388, Standard deviation: 20.95, CV(%): 5.4
- Sample 3: n=24, Mean(pg/ml): 709, Standard deviation: 34.74, CV(%): 4.9
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all FGF19 ELISA试剂盒
- FGF19 (Fibroblast Growth Factor 19 (FGF19))
- 别名
- FGF19 (FGF19 产品)
- 别名
- FGF-19 ELISA Kit, Fgf15 ELISA Kit, fibroblast growth factor 19 ELISA Kit, FGF19 ELISA Kit, Fgf19 ELISA Kit, fgf19 ELISA Kit
- 背景
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Protein Function: Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4. .
Background: FGF19, Fibroblast growth factor 19, is a protein that in humans is encoded by the FGF19 gene. The protein encoded by this gene is a member of the fibroblast growth factor(FGF) family. The FGF19 gene is mapped to 11q13.3. The deduced 216-amino acid FGF19 protein contains asignal sequence and 2 cysteine residues that are conserved in the FGF family. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development. FGF19 stimulates hepatic protein and glycogen synthesis but does not induce lipogenesis. The effects of FGF19 are independent of the activity of either insulin or the protein kinase Akt and, instead, are mediated through a mitogen-activated protein kinase signaling pathway that activates components of the protein translation machinery and stimulates glycogen synthase activity.
Synonyms: Fibroblast growth factor 19,FGF-19,FGF19,UNQ334/PRO533,
Full Gene Name: Fibroblast growth factor 19
Cellular Localisation: Secreted. - 基因ID
- 9965
- UniProt
- O95750
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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