Kallikrein 14 ELISA 试剂盒

Sequence similarities: Belongs to the peptidase S1 family. Kallikrein subfamily.

Tissue Specificity: Highly expressed in CNS, bone marrow and fetal liver. Also expressed in breast, thyroid, kidney, colon, pancreas, spleen, prostate, uterus, small intestine, placenta and skeletal muscle. Among 40 tissues tested, the highest expression is detected in skin followed by breast and prostate (at protein level). Expressed in stratum corneum by sweat ducts and sweat glands and detected in sweat (at protein level). .

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human Kallikrein 14 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, serum, plasma(heparin, EDTA) or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Kallikrein 14 standard solution and each sample be measured in duplicate.

• Sample 1: n=16, Mean(pg/ml): 124, Standard deviation: 8.31, CV(%): 6.7
• Sample 2: n=16, Mean(pg/ml): 364, Standard deviation: 17.5, CV(%): 4.8
• Sample 3: n=16, Mean(pg/ml): 613, Standard deviation: 32.5, CV(%): 5.3,
• Sample 1: n=24, Mean(pg/ml): 146, Standard deviation: 10.66, CV(%): 7.3
• Sample 2: n=24, Mean(pg/ml): 408, Standard deviation: 22.44, CV(%): 5.5
• Sample 3: n=24, Mean(pg/ml): 644, Standard deviation: 39.3, CV(%): 6.1

Protein Function: Serine-type endopeptidase with a dual trypsin-like and chymotrypsin-like substrate specificity. May activate/inactivate the proteinase-activated receptors F2R, F2RL1 and F2RL3 and other kallikreins including KLK1, KLK3, KLK5 and KLK11. May function in seminal clot liquefaction through direct cleavage of the semenogelin SEMG1 and SEMG2 and activation of KLK3. May function through desmoglein DSG1 cleavage in epidermal desquamation a process by which the most superficial corneocytes are shed from the skin surface. May be involved in several aspects of tumor progression including growth, invasion and angiogenesis. .

Background: Kallikrein-14 is a protein that in humans is encoded by the KLK14 gene. Kallikrein-14 belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. Kallikrein-14 gene is identified in the kallikrein gene cluster on chromosome 19q13.4. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. Kallikrein-14 may activate or inactivate the proteinase-activated receptors F2R, F2RL1 and F2RL3 and other kallikreins including KLK1, KLK3, KLK5 and KLK11.

Synonyms: Kallikrein-14,hK14,3.4.21.-,Kallikrein-like protein 6,KLK-L6,KLK14,KLKL6,

Full Gene Name: Kallikrein-14