Kallikrein 6 ELISA 试剂盒
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Quick Overview for Kallikrein 6 ELISA 试剂盒 (ABIN1889322)
抗原
See all Kallikrein 6 (KLK6) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 78 pg/mL
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原理
- Sandwich Human Kallikrein-6 / KLK6 ELISA Kit to quantitate Human KLK6 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: L22-K244Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 3 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Kallikrein 6 (KLK6) ELISA Kit
KLK6 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Kallikrein 6 (KLK6) ELISA KitKLK6 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cerebrospinal Fluid, Milk, Plasma, Serum
Kallikrein 6 (KLK6) ELISA KitKLK6 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cerebrospinal Fluid, Milk, Plasma, Serum, Tissue Homogenate
Kallikrein 6 (KLK6) ELISA KitKLK6 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Kallikrein 6 (KLK6) ELISA KitKLK6 适用: 人 Colorimetric Competition ELISA 1.52 pg/mL - 123.5 pg/mL Cerebrospinal Fluid, Milk, Plasma, Serum, Tissue Homogenate
Kallikrein 6 (KLK6) ELISA KitKLK6 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cerebrospinal Fluid, Milk, Plasma, Serum, Tissue Homogenate
Kallikrein 6 (KLK6) ELISA KitKLK6 适用: 人 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Kallikrein 6 (KLK6) ELISA KitKLK6 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cerebrospinal Fluid, Milk, Plasma, Serum
Kallikrein 6 (KLK6) ELISA KitKLK6 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cerebrospinal Fluid, Milk, Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 7.1%, 5.6%, 5.5%
Inter-Assay Precision (CV%): 7.6%, 6.7%, 7.2% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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: "Specific microRNA-mRNA Regulatory Network of Colon Cancer Invasion Mediated by Tissue Kallikrein-Related Peptidase 6." in: Neoplasia (New York, N.Y.), Vol. 19, Issue 5, pp. 396-411, (2018) (PubMed).
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- Kallikrein 6 (KLK6)
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别名
- KLK6
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背景
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Background: KLK6 (Kallikrein-related peptidase 6), also called KALLIKREIN 6, NEUROSIN, PROTEASE M, ZYME or PRSS9, is a protein that in humans is encoded by the KLK6 gene. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. The encoded enzyme is regulated by steroid hormones. Northern blot analysis revealed that the PRSS9 mRNA was expressed in several primary tumors and cell lines from mammary, prostate, and ovarian cancers, but was not detected in any metastases of these cancers. The KLK6 gene is mapped on 19q13.41. In tissue culture, the enzyme has been found to generate amyloidogenic fragments from the amyloid precursor protein, suggesting a potential for involvement in Alzheimer's disease. Upon cellular stress, neurosin was released from mitochondria to the cytosol, which resulted in the increase of degraded alpha-synuclein species. Neurosin may play a significant role in physiologic alpha-synuclein degradation and also in the pathogenesis of synucleinopathies.
Gene Full Name: kallikrein related peptidase 6
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基因ID
- 5653
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UniProt
- Q92876
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途径
- Complement System, Regulation of G-Protein Coupled Receptor Protein Signaling
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