IGFBP2 ELISA 试剂盒
Quick Overview for IGFBP2 ELISA 试剂盒 (ABIN1889295)
抗原
See all IGFBP2 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 125 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IGFBP2
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: E35-Q305 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: E35-Q305
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Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2) ELISA Kit
IGFBP2 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate, Urine
Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2) ELISA KitIGFBP2 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2) ELISA KitIGFBP2 适用: Pig Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2) ELISA KitIGFBP2 适用: 大鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2) ELISA KitIGFBP2 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2) ELISA KitIGFBP2 适用: 小鸡 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma, Serum
Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2) ELISA KitIGFBP2 适用: Pig Colorimetric Sandwich ELISA 0.46 ng/mL - 30 ng/mL Plasma, Serum
Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2) ELISA KitIGFBP2 适用: Cow Colorimetric Sandwich ELISA 0.94 ng/mL - 60 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2) ELISA KitIGFBP2 适用: 人 AA 40-328 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2) ELISA KitIGFBP2 适用: 犬 Colorimetric Competition ELISA 0.5-10 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Contains 1 IGFBP N-terminal domain.
Tissue Specificity: Highly expressed in adult liver, but also in kidney, lung, brain, spleen, testis and ovary. .
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板类型
- Pre-coated
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实验流程
- mouse IGFBP2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IGFBP2 has been precoated onto 96-well plates. Standards(NSO, E35-Q305) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IGFBP2 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL mouse IGFBP2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IGFBP2 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 1257, Standard deviation: 89.2, CV(%): 7.1
- Sample 2: n=16, Mean(pg/ml): 3204, Standard deviation: 166.6, CV(%): 5.2
- Sample 3: n=16, Mean(pg/ml): 5394, Standard deviation: 350.61, CV(%): 6.5,
- Sample 1: n=24, Mean(pg/ml): 1020, Standard deviation: 84.66, CV(%): 8.3
- Sample 2: n=24, Mean(pg/ml): 3562, Standard deviation: 239, CV(%): 6.7
- Sample 3: n=24, Mean(pg/ml): 4975, Standard deviation: 368.15, CV(%): 7.4
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- IGFBP2 (Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))
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别名
- IGFBP2
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背景
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Protein Function: Inhibits IGF-mediated growth and developmental rates (By similarity). IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. .
Background: Insulin-like growth factor-binding protein 2(IGFBP2), also called IBP2, is a protein that in humans is encoded by the IGFBP2 gene. It is mapped to 2q35. By structural analysis, it showed that the IGFBP2 gene consists of 4 exons with 3 introns of lengths 27.0, 1.0, and 1.9 kb. IGFBP2 secreted by metastatic cells recruits endothelia by modulating IGF1 mediated activation of the IGF type-I receptor on endothelial cells. The IGFBP2/IGF1/IGF1R and GAS6/MERTK signaling pathways are regulators of cancer-mediated endothelial recruitment.
Synonyms: Insulin-like growth factor-binding protein 2,IBP-2,IGF-binding protein 2,IGFBP-2,mIGFBP-2,Igfbp2,Igfbp-2,
Full Gene Name: Insulin-like growth factor-binding protein 2
Cellular Localisation: Secreted. -
基因ID
- 16008
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UniProt
- P47877
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途径
- Myometrial Relaxation and Contraction, Growth Factor Binding, Activated T Cell Proliferation
抗原 See all IGFBP2 ELISA试剂盒
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