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Quick Overview for SensoLyte® 520 MMP-2 Assay Kit (ABIN1882519)
抗原
See all MMP2 试剂盒检测方法
应用范围
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产品特性
- SensoLyte® 520 MMP-2 Assay Kit uses a 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRET peptide by MMP-2, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.
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Matrix Metalloproteinase 2 (MMP2) ELISA Kit
MMP2 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: Pig Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: 马 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: 大鼠 AA 30-662 Colorimetric Sandwich ELISA 625-40000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: 人 AA 30-660 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: 小鼠 AA 30-662 Colorimetric Sandwich ELISA 625-40000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: 人 Colorimetric Sandwich ELISA 0.5 ng/mL - 32 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: Pig Colorimetric Sandwich ELISA 47 ng/mL - 3000 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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说明
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FRET-based Assay Kit
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限制
- 仅限研究用
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注意事项
- Protect Components A and B from light.
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储存条件
- -20 °C
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储存方法
- Store all components at -20 °C. Components D and E can be stored at 4 °C for convenience.
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: "Endothelin A receptor antagonism enhances inhibitory effects of anti-ganglioside GD2 monoclonal antibody on invasiveness and viability of human osteosarcoma cells." in: PLoS ONE, Vol. 9, Issue 4, pp. e93576, (2014) (PubMed).
: "Matrix metalloproteinase content and activity in low-platelet, low-leukocyte and high-platelet, high-leukocyte platelet rich plasma (PRP) and the biologic response to PRP by human ligament ..." in: The American journal of sports medicine, Vol. 42, Issue 5, pp. 1211-8, (2014) (PubMed).
: "Inhibition of matrix metalloproteinases prevents the potentiation of nondeprived-eye responses after monocular deprivation in juvenile rats." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 22, Issue 3, pp. 725-34, (2012) (PubMed).
: "ARP101, a selective MMP-2 inhibitor, induces autophagy-associated cell death in cancer cells." in: Biochemical and biophysical research communications, Vol. 404, Issue 4, pp. 1039-43, (2011) (PubMed).
: "Fluid shear stress regulates the invasive potential of glioma cells via modulation of migratory activity and matrix metalloproteinase expression." in: PLoS ONE, Vol. 6, Issue 5, pp. e20348, (2011) (PubMed).
: "A novel pharmacophore model for the design of anthrax lethal factor inhibitors." in: Chemical biology & drug design, Vol. 76, Issue 3, pp. 263-8, (2010) (PubMed).
: "Failure of pelvic organ support in mice deficient in fibulin-3." in: The American journal of pathology, Vol. 174, Issue 1, pp. 206-15, (2009) (PubMed).
: "Interstitial flow promotes vascular fibroblast, myofibroblast, and smooth muscle cell motility in 3-D collagen I via upregulation of MMP-1." in: American journal of physiology. Heart and circulatory physiology, Vol. 297, Issue 4, pp. H1225-34, (2009) (PubMed).
: "Quantification of pelvic organ prolapse in mice: vaginal protease activity precedes increased MOPQ scores in fibulin 5 knockout mice." in: Biology of reproduction, Vol. 80, Issue 3, pp. 407-14, (2009) (PubMed).
: "Regulation of elastolytic proteases in the mouse vagina during pregnancy, parturition, and puerperium." in: Biology of reproduction, Vol. 78, Issue 3, pp. 521-8, (2008) (PubMed).
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: "Endothelin A receptor antagonism enhances inhibitory effects of anti-ganglioside GD2 monoclonal antibody on invasiveness and viability of human osteosarcoma cells." in: PLoS ONE, Vol. 9, Issue 4, pp. e93576, (2014) (PubMed).
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- MMP2 (Matrix Metalloproteinase 2 (MMP2))
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别名
- MMP-2
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背景
- Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion is well known. MMP-2 (gelatinase A) is proposed as a potential therapeutic target.
抗原
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