(3 references)-
- 抗原 See all MMP12 试剂盒
- MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))
- 检测方法
- Fluorometric
- 应用范围
- Fluorescence Resonance Energy Transfer Microscopy (FRET)
- 品牌
- SensoLyte®
- 产品特性
- SensoLyte® 520 MMP-12 Assay Kit uses a 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. This substrate showed excellent specificity to MMP-12 with minimal cross-reaction with MMP-1, 2, 7, 8, 9, 9, 13 and 14. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRET peptide by MMP-12, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.
- Top Product
- Discover our top product MMP12 ELISA Kit
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Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA Kit
MMP12 适用: 大鼠 Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA KitMMP12 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA KitMMP12 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA KitMMP12 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA KitMMP12 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Homogenates, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA KitMMP12 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA KitMMP12 适用: 大鼠 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA KitMMP12 适用: 人 AA 17-470 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA KitMMP12 适用: 小鼠 AA 18-462 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA KitMMP12 适用: 人 Colorimetric Sandwich ELISA 0.625-40 ng/mL Plasma, Serum, Tissue Homogenate
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- 说明
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FRET-based Assay Kit
- 限制
- 仅限研究用
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- 注意事项
- Protect Components A and B from light.
- 储存条件
- -20 °C
- 储存方法
- Store all components at -20 °C. Components D and E can be stored at 4 °C for convenience.
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: "The endogenous protease inhibitor TIMP-1 mediates protection and recovery from cutaneous photodamage." in: The Journal of investigative dermatology, Vol. 132, Issue 12, pp. 2800-9, (2012) (PubMed).
: "Matrix metalloproteinase-12 (MMP-12) SNP affects MMP activity, lung macrophage infiltration and protects against emphysema in COPD." in: Thorax, Vol. 66, Issue 11, pp. 970-6, (2011) (PubMed).
: "Modulation of the angiogenic phenotype of normal and systemic sclerosis endothelial cells by gain-loss of function of pentraxin 3 and matrix metalloproteinase 12." in: Arthritis and rheumatism, Vol. 62, Issue 8, pp. 2488-98, (2010) (PubMed).
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: "The endogenous protease inhibitor TIMP-1 mediates protection and recovery from cutaneous photodamage." in: The Journal of investigative dermatology, Vol. 132, Issue 12, pp. 2800-9, (2012) (PubMed).
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- 抗原
- MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))
- 别名
- MMP-12 (MMP12 产品)
- 背景
- Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. MMP-12 (macrophage elastase) is expressed in activated macrophages and may play a role in smoke-induced emphysema.
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