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Quick Overview for SensoLyte® 520 MMP-1 Assay Kit (ABIN1882510)
抗原
See all MMP1 试剂盒检测方法
应用范围
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产品特性
- SensoLyte® 520 MMP-1 Assay Kit uses a 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET peptide substrate for continuous measurement of enzyme activity. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRET peptide by MMP-1, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide has less interference from the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format.
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SensoLyte® Plus 520 MMP-1 Assay Kit Fluorimetric and Enhanced Selectivity
FRET 适用: 人 Fluorometric
SensoLyte® 490 MMP-1 Assay KitFRET Fluorometric
Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) ELISA KitMMP1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) ELISA KitMMP1 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) ELISA KitMMP1 适用: 兔 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) ELISA KitMMP1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) ELISA KitMMP1 适用: Cow Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) ELISA KitMMP1 适用: 人 AA 20-469 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) ELISA KitMMP1 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) ELISA KitMMP1 适用: 小鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
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说明
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FRET-based Assay Kit
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限制
- 仅限研究用
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注意事项
- Protect Components A and B from light.
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储存条件
- -20 °C
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储存方法
- Store all components at -20 °C. Components D and E can be stored at 4 °C for convenience.
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: "Matrix metalloproteinase (MMP)-1 induces lung alveolar epithelial cell migration and proliferation, protects from apoptosis, and represses mitochondrial oxygen consumption." in: The Journal of biological chemistry, Vol. 288, Issue 36, pp. 25964-75, (2013) (PubMed).
: "Liver regeneration associated protein (ALR) exhibits antimetastatic potential in hepatocellular carcinoma." in: Molecular medicine (Cambridge, Mass.), Vol. 17, Issue 3-4, pp. 221-8, (2011) (PubMed).
: "Matrix metalloproteinase-1 induces cleavage of exogenous alphaB-crystallin transduced by a cell-penetrating peptide." in: Journal of cellular biochemistry, Vol. 112, Issue 9, pp. 2454-62, (2011) (PubMed).
: "Development of a gene therapy virus with a glucocorticoid-inducible MMP1 for the treatment of steroid glaucoma." in: Investigative ophthalmology & visual science, Vol. 51, Issue 6, pp. 3029-41, (2010) (PubMed).
: "Inhibition of ceramide-redox signaling pathway blocks glomerular injury in hyperhomocysteinemic rats." in: Kidney international, Vol. 70, Issue 1, pp. 88-96, (2006) (PubMed).
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: "Matrix metalloproteinase (MMP)-1 induces lung alveolar epithelial cell migration and proliferation, protects from apoptosis, and represses mitochondrial oxygen consumption." in: The Journal of biological chemistry, Vol. 288, Issue 36, pp. 25964-75, (2013) (PubMed).
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- MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))
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别名
- MMP-1
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背景
- MMP-1 (interstitial collagenase, fibroblast collagenase) is an extracellular protease. This enzyme plays a significant role in cancer, arthritis and other diseases, and has been identified as a drug-screening target.
抗原
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