MMP1 ELISA 试剂盒
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Quick Overview for MMP1 ELISA 试剂盒 (ABIN411327)
抗原
See all MMP1 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich Human MMP-1 ELISA Kit to quantitate Human MMP1 in cell culture supernatants, serum and plasma (heparin).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: F20-N469Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 20 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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SensoLyte® Plus 520 MMP-1 Assay Kit Fluorimetric and Enhanced Selectivity
FRET 适用: 人 Fluorometric
SensoLyte® 520 MMP-1 Assay KitFRET Fluorometric
SensoLyte® 490 MMP-1 Assay KitFRET Fluorometric
Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) ELISA KitMMP1 适用: 兔 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) ELISA KitMMP1 适用: Cow Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) ELISA KitMMP1 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) ELISA KitMMP1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) ELISA KitMMP1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) ELISA KitMMP1 适用: 犬 Colorimetric Sandwich ELISA 0.18 ng/mL - 12 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) ELISA KitMMP1 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 6.5%, 7.6%, 5.8%
Inter-Assay Precision (CV%): 8.3%, 9.4%, 7.6% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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: "MALAT1 participates in ultraviolet B-induced photo-aging via regulation of the ERK/MAPK signaling pathway." in: Molecular medicine reports, Vol. 15, Issue 6, pp. 3977-3982, (2018) (PubMed).
: "Ganoderma lucidum polysaccharides protect fibroblasts against UVB-induced photoaging." in: Molecular medicine reports, Vol. 15, Issue 1, pp. 111-116, (2017) (PubMed).
: "Salivary MMP-1, MMP-2, MMP-3 and MMP-13 Levels in Patients with Oral Lichen Planus and Squamous Cell Carcinoma" in: Asian Pacific journal of cancer prevention : APJCP, Vol. 18, Issue 7, pp. 1947-1951, (2017) (PubMed).
: "In vitro response of human peripheral blood mononuclear cells to AISI 316L austenitic stainless steel subjected to nitriding and collagen coating treatments." in: Journal of materials science. Materials in medicine, Vol. 26, Issue 2, pp. 100, (2015) (PubMed).
: "Decreased expression of matrix metalloproteinase-1 in the maternal umbilical serum, trophoblasts and decidua leads to preeclampsia." in: Experimental and therapeutic medicine, Vol. 9, Issue 3, pp. 992-998, (2015) (PubMed).
: "Tuning composition and architecture of biomimetic scaffolds for enhanced matrix synthesis by murine cardiomyocytes." in: Journal of biomedical materials research. Part A, Vol. 103, Issue 2, pp. 693-708, (2015) (PubMed).
: "Serum Levels of IL-1 ? , IL-6, TGF- ? , and MMP-9 in Patients Undergoing Carotid Artery Stenting and Regulation of MMP-9 in a New In Vitro Model of THP-1 Cells Activated by Stenting." in: Mediators of inflammation, Vol. 2015, pp. 956082, (2015) (PubMed).
: "Effects of Elaeagnus angustifolia L. supplementation on serum levels of inflammatory cytokines and matrix metalloproteinases in females with knee osteoarthritis." in: Complementary therapies in medicine, Vol. 22, Issue 5, pp. 864-9, (2014) (PubMed).
: "Tumor necrosis factor-?-accelerated degradation of type I collagen in human skin is associated with elevated matrix metalloproteinase (MMP)-1 and MMP-3 ex vivo." in: European journal of cell biology, Vol. 94, Issue 1, pp. 12-21, (2014) (PubMed).
: "Study of the correlations among some parameters of the oxidative status, gelatinases, and their inhibitors in a group of subjects with metabolic syndrome." in: Mediators of inflammation, Vol. 2014, pp. 510619, (2014) (PubMed).
: "Gelatinases and their tissue inhibitors in a group of subjects with metabolic syndrome." in: Journal of investigative medicine : the official publication of the American Federation for Clinical Research, Vol. 61, Issue 6, pp. 978-83, (2013) (PubMed).
: "Implication of MMP-9 and urokinase plasminogen activator (uPA) in the activation of pro-matrix metalloproteinase (MMP)-13." in: Rheumatology international, Vol. 32, Issue 10, pp. 3069-75, (2012) (PubMed).
: "Detection and comparison of matrix metalloproteinase in primary and recurrent pterygium fibroblasts." in: International journal of ophthalmology, Vol. 4, Issue 4, pp. 353-6, (2012) (PubMed).
: "IPL irradiation rejuvenates skin collagen via the bidirectional regulation of MMP-1 and TGF-?1 mediated by MAPKs in fibroblasts." in: Lasers in medical science, Vol. 26, Issue 3, pp. 381-7, (2011) (PubMed).
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- MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))
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别名
- MMP1
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背景
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Background: Matrix metalloproteinase-1 (MMP-1) is also known as collagenase. Matrix metalloproteinases (MMPs) are zinc-dependent proteases that degrade extrMMP-1llular matrix proteins. The MMPs comprise a family of at least 20 proteolytic enzymes that play an essential role in tissue remodeling. Matrix metalloproteinase-1 (MMP-1) plays an important role in the degradation of collagen in inflammatory diseases. MMP1 (interstitial collagenase), MMP9 (gelatinase B) and MMP12 (macrophage elastase) are thought to be important in the development of emphysema. Smoking-induced MMP-1 might be important in the skin-ageing effects of tobacco smoking. The standard product used in this kit is human MMP-1 with the molecular mass of 51-53KDa. The detected MMP-1 includes zymogen and active enzyme.
Gene Full Name: matrix metallopeptidase 1
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基因ID
- 4312
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UniProt
- P03956
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