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SensoLyte® Homogeneous AMC Caspase-3/7 Assay Kit

Name: SensoLyte® Homogeneous AMC Caspase-3/7 Assay Kit
SKU: ABIN1882431
Availability: OutOfStock

FRET Fluorometric

ELISA

1 image

(32 references)

产品编号 ABIN1882431

发货至: 中国

Quick Overview for SensoLyte® Homogeneous AMC Caspase-3/7 Assay Kit (ABIN1882431)

抗原

See all Caspase 3 (CASP3) 试剂盒

Caspase 3 (CASP3)

检测方法

Fluorometric

应用范围

Fluorescence Resonance Energy Transfer Microscopy (FRET)

Discover our top product CASP3 ELISA Kit

品牌

SensoLyte®

产品特性

The SensoLyte®Homogeneous AMC Capase-3/7 Assay Kit uses Ac-DEVD-AMC as the fluorogenic indicator for assaying caspase-3/7 activities. Upon caspase-3/7 cleavage, Ac-DEVD-AMC generates the AMC fluorophore which has bright blue fluorescence and can be detected at Ex/Em=354 nm/442 nm. A bi-function assay buffer in this kit is designed to lyze the cells and measure the enzyme activity at the same time. Thus, this kit can measure caspase-3/7 activity in cell culture directly in a 96-well or 384-well plate without a time-consuming cell extraction step. In case the cells are cultured in larger plates or flasks, a lysis buffer and protocol for cell lysate preparation are also conveniently included in the kit. The kit is suitable for high throughput screening of apoptosis inducers and inhibitors.
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说明

FRET-based Assay Kit

限制

仅限研究用
注意事项

Keep Components A and B away from light

储存条件

-20 °C
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抗原

Caspase 3 (CASP3)

别名

Caspase-3

背景

Central to the execution phase of apoptosis are the two closely related caspase-3 and caspase-7. They share common substrate specificity and structure, but differ completely in the sequence of their respective N-terminal regions. Both caspase-3 and caspase-7 have substrate selectivity for the amino acid sequence Asp-Glu-Val-Asp (DEVD).