(3 references)Quick Overview for SensoLyte® AMC Calpain Activity Assay Kit (ABIN1882424)
抗原
Calpain
检测方法
Fluorometric
应用范围
Activation (Act), Fluorescence Resonance Energy Transfer Microscopy (FRET)
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品牌
- SensoLyte®
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产品特性
- The SensoLyte® AMC Calpain Assay Kit is optimized for detecting calpain activities. This kit contains a fluorogenic peptide substrate, suc-LLVY-AMC. Upon calpain protease cleavage, suc-LLVY-AMC generates the AMC fluorophore emitting bright blue fluorescence and can be monitored at excitation/emission=354/442 nm. Increase in AMC fluorescence is proportional to calpain activity. The assay can detect both calpain 1 (µ) and 2 (m) activities and is ideal for kinetic study of these enzymes. The assays are performed in a convenient 96-well microplate format.
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SensoLyte® 520 Calpain Activity Assay Kit
Act, FRET Fluorometric
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说明
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FRET-based Assay Kit
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限制
- 仅限研究用
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注意事项
- Aliquot as needed to avoid multiple freeze-thaw cycles. Protect Components A and B from light and moisture.
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储存条件
- -80 °C
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储存方法
- Store all kit components, except Component D, at -20 °C. Store Component D at -80 °C. Component C can be stored at room temperature for convenience.
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: "Calpain mediates epithelial cell microvillar effacement by enterohemorrhagic Escherichia coli." in: Frontiers in microbiology, Vol. 2, pp. 222, (2011) (PubMed).
: "Regulation of the calpain and ubiquitin-proteasome systems in a canine model of muscular dystrophy." in: Muscle & nerve, Vol. 44, Issue 4, pp. 553-62, (2011) (PubMed).
: "The JAMM motif of human deubiquitinase Poh1 is essential for cell viability." in: Molecular cancer therapeutics, Vol. 6, Issue 1, pp. 262-8, (2007) (PubMed).
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: "Calpain mediates epithelial cell microvillar effacement by enterohemorrhagic Escherichia coli." in: Frontiers in microbiology, Vol. 2, pp. 222, (2011) (PubMed).
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- Calpain
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背景
- The calpains are a family of intracellular Ca2+ supdependent cysteine proteases. The best-characterized calpains are isoforms 1 (m) and 2 (m). Calpains respond to Ca2+ signals by cleaving many specific proteins, thereby irreversibly modifying their function(s). They are implicated in a variety of Ca2+ regulated cellular processes as well as various pathological phenomena, such as ischemic injury, muscular dystrophy, diabetes, cataract, atherosclerosis, Alzheimer's disease, and cancer. Calpains represent potential therapeutic targets for drug discovery.
抗原
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