(3 references)-
- 抗原 See all Calpain products
- Calpain
- 检测方法
- Fluorometric
- 应用范围
- Activation (Act), Fluorescence Resonance Energy Transfer Microscopy (FRET)
- 品牌
- SensoLyte®
- 产品特性
- The SensoLyte® AMC Calpain Assay Kit is optimized for detecting calpain activities. This kit contains a fluorogenic peptide substrate, suc-LLVY-AMC. Upon calpain protease cleavage, suc-LLVY-AMC generates the AMC fluorophore emitting bright blue fluorescence and can be monitored at excitation/emission=354/442 nm. Increase in AMC fluorescence is proportional to calpain activity. The assay can detect both calpain 1 (µ) and 2 (m) activities and is ideal for kinetic study of these enzymes. The assays are performed in a convenient 96-well microplate format.
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SensoLyte® 520 Calpain Activity Assay Kit
Act, FRET Fluorometric
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- 说明
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FRET-based Assay Kit
- 限制
- 仅限研究用
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- 注意事项
- Aliquot as needed to avoid multiple freeze-thaw cycles. Protect Components A and B from light and moisture.
- 储存条件
- -80 °C
- 储存方法
- Store all kit components, except Component D, at -20 °C. Store Component D at -80 °C. Component C can be stored at room temperature for convenience.
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: "Calpain mediates epithelial cell microvillar effacement by enterohemorrhagic Escherichia coli." in: Frontiers in microbiology, Vol. 2, pp. 222, (2011) (PubMed).
: "Regulation of the calpain and ubiquitin-proteasome systems in a canine model of muscular dystrophy." in: Muscle & nerve, Vol. 44, Issue 4, pp. 553-62, (2011) (PubMed).
: "The JAMM motif of human deubiquitinase Poh1 is essential for cell viability." in: Molecular cancer therapeutics, Vol. 6, Issue 1, pp. 262-8, (2007) (PubMed).
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: "Calpain mediates epithelial cell microvillar effacement by enterohemorrhagic Escherichia coli." in: Frontiers in microbiology, Vol. 2, pp. 222, (2011) (PubMed).
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- 抗原
- Calpain
- Abstract
- Calpain 产品
- 背景
- The calpains are a family of intracellular Ca2+ supdependent cysteine proteases. The best-characterized calpains are isoforms 1 (m) and 2 (m). Calpains respond to Ca2+ signals by cleaving many specific proteins, thereby irreversibly modifying their function(s). They are implicated in a variety of Ca2+ regulated cellular processes as well as various pathological phenomena, such as ischemic injury, muscular dystrophy, diabetes, cataract, atherosclerosis, Alzheimer's disease, and cancer. Calpains represent potential therapeutic targets for drug discovery.
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