(5 references)-
- 抗原 See all Calpain products
- Calpain
- 检测方法
- Fluorometric
- 应用范围
- Activation (Act), Fluorescence Resonance Energy Transfer Microscopy (FRET)
- 品牌
- SensoLyte®
- 产品特性
- The SensoLyte® 520 Calpain Assay Kit is optimized for detecting calpain activity. This kit contains a novel internally quenched 5-FAM/QXL™ 520 FRET substrate. Calpain protease cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence which can be monitored at excitation/emission= 490/520 nm. Increase in fluorescence is proportional to the calpain activity. The assay can detect both calpain 1 (µ) and 2 (m) activities and is ideal for kinetic study of these enzymes. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. The assays are performed in a convenient 96-well microplate format.
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SensoLyte® AMC Calpain Activity Assay Kit
Act, FRET Fluorometric
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- 说明
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FRET-based Assay Kit
- 限制
- 仅限研究用
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- 注意事项
- Aliquot as needed to avoid multiple freeze-thaw cycles. Protect Components A and B from light and moisture.
- 储存条件
- -80 °C
- 储存方法
- Store all kit components, except Component D, at -20 °C. Store Component D at -80 °C. Component C can be stored at room temperature for convenience.
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: "Effect of partial bladder outlet obstruction and reversal on rabbit bladder physiology and biochemistry: duration of recovery period and severity of function." in: BJU international, Vol. 114, Issue 6, pp. 946-54, (2014) (PubMed).
: "Targeted disruption of Nrg1 in granulosa cells alters the temporal progression of oocyte maturation." in: Molecular endocrinology (Baltimore, Md.), Vol. 28, Issue 5, pp. 706-21, (2014) (PubMed).
: "The effect of partial outlet obstruction on calpain and phospholipase-2 activities: analyzed by severity and duration." in: Molecular and cellular biochemistry, Vol. 381, Issue 1-2, pp. 217-20, (2013) (PubMed).
: "EGF-like factors induce expansion of the cumulus cell-oocyte complexes by activating calpain-mediated cell movement." in: Endocrinology, Vol. 153, Issue 8, pp. 3949-59, (2012) (PubMed).
: "Developmentally regulated ceramide synthase 6 increases mitochondrial Ca2+ loading capacity and promotes apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 6, pp. 4644-58, (2011) (PubMed).
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: "Effect of partial bladder outlet obstruction and reversal on rabbit bladder physiology and biochemistry: duration of recovery period and severity of function." in: BJU international, Vol. 114, Issue 6, pp. 946-54, (2014) (PubMed).
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- 抗原
- Calpain
- Abstract
- Calpain 产品
- 别名
- CG18152 Kit, CG7563 Kit, Dmel\\CG7563 Kit, EST C Kit, calpain Kit, Tb08.28A12.350 Kit, Calpain-A Kit, hypothetical protein Kit, calpain 11 Kit, calpain Kit, CalpA Kit, T11A5.6 Kit, CAPN11 Kit, Tb927.8.8330 Kit
- 背景
- The calpains are a family of intracellular Ca2+ dependent cysteine proteases. The best-characterized calpains are isoforms 1 (µ) and 2 (m). They respond to Ca2+ signals by cleaving many specific proteins, thereby irreversibly modifying their function(s). Calpains are implicated in a variety of Ca2+ regulated cellular processes as well as various pathological phenomena such as ischemic injury, muscular dystrophy, diabetes, cataract, atherosclerosis, Alzheimer's disease, and cancer. Calpains represent potential therapeutic targets for drug discovery.
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