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MMP12 ELISA 试剂盒

Name: MMP12 ELISA 试剂盒
SKU: ABIN1706069
Availability: OutOfStock

This 人 MMP12 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 MMP12. This ELISA Kit has been cited in 3+ publications.

ELISA

1 image

(3 references)

产品编号 ABIN1706069

发货至: 中国

Quick Overview for MMP12 ELISA 试剂盒 (ABIN1706069)

抗原

See all MMP12 ELISA试剂盒

MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))

抗原表位

AA 17-470

适用

人

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

62.5-4000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin)

Discover our top product MMP12 ELISA Kit

最低检测浓度

62.5 pg/mL

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MMP-12

品牌

PicoKine™

Analytical Method

Quantitative

特异性

Expression system for standard: NSO
Immunogen sequence: L17-C470

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<10pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: NSO
Immunogen sequence: L17-C470
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA Kit
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Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA Kit
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Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA Kit
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Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA Kit
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Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA Kit
MMP12 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

说明

Sequence similarities: Belongs to the peptidase M10A family.
Tissue Specificity: Found in alveolar macrophages but not in peripheral blood monocytes.

板类型

Pre-coated

实验流程

human MMP-12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MMP-12 has been precoated onto 96-well plates. Standards(NSO, L17-C470) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MMP-12 amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human MMP-12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MMP-12 standard solution and each sample be measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(pg/ml): 478, Standard deviation: 28.68, CV(%): 6
- Sample 2: n=16, Mean(pg/ml): 1512, Standard deviation: 84.7, CV(%): 5.6
- Sample 3: n=16, Mean(pg/ml): 2388, Standard deviation: 107.5, CV(%): 4.5,
- Sample 1: n=24, Mean(pg/ml): 526, Standard deviation: 37.9, CV(%): 7.2
- Sample 2: n=24, Mean(pg/ml): 1724, Standard deviation: 113.8, CV(%): 6.6
- Sample 3: n=24, Mean(pg/ml): 2653, Standard deviation: 143.3, CV(%): 5.4
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
Bchir, Nasr, Bouchet, Benzarti, Garrouch, Tabka, Susin, Chahed, Bauvois: "Concomitant elevations of MMP-9, NGAL, proMMP-9/NGAL and neutrophil elastase in serum of smokers with chronic obstructive pulmonary disease." in: Journal of cellular and molecular medicine, Vol. 21, Issue 7, pp. 1280-1291, (2018) (PubMed).

Narimiya, Wada, Kanzaki, Ishikawa, Tsuge, Yamaguchi, Nakamura: "Orthodontic tensile strain induces angiogenesis via type IV collagen degradation by matrix metalloproteinase-12." in: Journal of periodontal research, Vol. 52, Issue 5, pp. 842-852, (2018) (PubMed).

Kobayashi, Jokaji, Miyazawa-Hira, Takatsuka, Tanaka, Ooi, Nakamura, Kawashiri: "Elastin‑derived peptides are involved in the processes of human temporomandibular disorder by inducing inflammatory responses in synovial cells." in: Molecular medicine reports, Vol. 16, Issue 3, pp. 3147-3154, (2018) (PubMed).
抗原 See all MMP12 ELISA试剂盒

MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))

别名

MMP12

背景

Protein Function: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
Background: Matrix metalloproteinase-12(MMP12), also known as MME or ME, is an enzyme that in humans is encoded by the MMP12 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.2. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. This gene may involved in tissue injury and remodeling.
Synonyms: Macrophage metalloelastase,MME,3.4.24.65,Macrophage elastase,ME,hME,Matrix metalloproteinase-12,MMP-12,MMP12,HME,
Full Gene Name: Macrophage metalloelastase
Cellular Localisation: Secreted, extracellular space, extracellular matrix.

基因ID

4321

UniProt

P39900