Neurotrophin 4 ELISA 试剂盒
(2 references)Our Local Distributor
北京 101111
Quick Overview for Neurotrophin 4 ELISA 试剂盒 (ABIN1706065)
抗原
See all Neurotrophin 4 (NTF4) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 31.2 pg/mL
-
原理
- Sandwich Human NT-4 ELISA Kit to quantitate Human NTF4 in cell culture supernatants and serum.
-
Analytical Method
- Quantitative
-
特异性
-
Expression system for standard: NS0
Immunogen sequence: G81-A210Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
-
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
-
灵敏度
- < 10 pg/mL
-
组件
-
- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
-
试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
-
-
Neurotrophin 4 (NTF4) ELISA Kit
NTF4 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neurotrophin 4 (NTF4) ELISA KitNTF4 适用: 人 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Plasma, Serum, Tissue Homogenate
Neurotrophin 4 (NTF4) ELISA KitNTF4 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Neurotrophin 4 (NTF4) ELISA KitNTF4 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Neurotrophin 4 (NTF4) ELISA KitNTF4 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neurotrophin 4 (NTF4) ELISA KitNTF4 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Neurotrophin 4 (NTF4) ELISA KitNTF4 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neurotrophin 4 (NTF4) ELISA KitNTF4 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neurotrophin 4 (NTF4) ELISA KitNTF4 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neurotrophin 4 (NTF4) ELISA KitNTF4 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Serum
-
-
应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
-
样本量
- 100 μL
-
板类型
- Pre-coated
-
实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
-
实验精密度
-
Intra-Assay Precision (CV%): 6.4%, 5.7%, 7.8%
Inter-Assay Precision (CV%): 6.4%, 6.9%, 9.7% -
限制
- 仅限研究用
-
-
-
注意事项
- Avoid multiple freeze-thaw cycles.
-
储存条件
- -20 °C,4 °C
-
储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
-
有效期
- 12 months
-
-
-
: "Live attenuated Salmonella displaying HIV-1 10E8 epitope on fimbriae: systemic and mucosal immune responses in BALB/c mice by mucosal administration." in: Scientific reports, Vol. 6, pp. 29556, (2018) (PubMed).
: "Interaction between neurotrophin 4 and gonadotrophin in bovine oviducts." in: Theriogenology, Vol. 78, Issue 1, pp. 39-48, (2012) (PubMed).
-
-
- Neurotrophin 4 (NTF4)
-
别名
- NTF4
-
背景
-
Background: Neurotrophin-4 (NT-4), also known as neurotrophin-5 (NT-5), is a protein that in humans is encoded by the NTF4 gene. Human NT-4 as well as a human NT-4 pseudogene colocalize to chromosome 19 band q13.3. NT-4 is a member of a family of neurotrophic factors, the neurotrophins, that control survival and differentiation of vertebrate neurons (2-4). NT-4 is a neurotrophic factor that signals predominantly through the TrkB receptor tyrosine kinase. NT4 plays a physiological role essential for hippocampus- and amygdala-dependent long-term memory and hippocampal long-lasting LTP and that NT4 may be useful in the therapy of acquired disorders of learning and memory.
Gene Full Name: neurotrophin 4
-
基因ID
- 4909
-
UniProt
- P34130
-
途径
- RTK signaling
抗原 See all Neurotrophin 4 (NTF4) ELISA试剂盒
-
