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Neurotrophin 4 ELISA 试剂盒

NTF4 适用: 人 AA 81-210 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Serum
产品编号 ABIN1706065
发货至: 中国
  • 抗原 See all Neurotrophin 4 (NTF4) ELISA试剂盒
    Neurotrophin 4 (NTF4)
    抗原表位
    AA 81-210
    适用
    • 3
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    31.2-2000 pg/mL
    最低检测浓度
    31.2 pg/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human NT-4
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性
    Expression system for standard: sf21
    Immunogen sequence: G81-A210
    交叉反应 (详细)
    There is no detectable cross-reactivity with other relevant proteins.
    灵敏度
    <10pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Expression system for standard: sf21
    Immunogen sequence: G81-A210
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  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    说明

    Tissue Specificity: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues.

    板类型
    Pre-coated
    实验流程
    human NT-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for NT-4 has been precoated onto 96-well plates. Standards(sf21, G81-A210) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NT-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human NT-4 amount of sample captured in plate.
    实验流程

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human NT-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human NT-4 standard solution and each sample be measured in duplicate.

    实验精密度
    • Sample 1: n=16, Mean(pg/ml): 248, Standard deviation: 11.16, CV(%): 4.5
    • Sample 2: n=16, Mean(pg/ml): 745, Standard deviation: 38, CV(%): 5.1
    • Sample 3: n=16, Mean(pg/ml): 1243, Standard deviation: 78.31, CV(%): 6.3,
    • Sample 1: n=24, Mean(pg/ml): 280, Standard deviation: 14.84, CV(%): 5.3
    • Sample 2: n=24, Mean(pg/ml): 821, Standard deviation: 46, CV(%): 5.6
    • Sample 3: n=24, Mean(pg/ml): 1402, Standard deviation: 96.74, CV(%): 6.9
    限制
    仅限研究用
  • 注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    -20 °C,4 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • Li, Jin, Wang, Li, Liu, Chang, Wang, Liu: "Live attenuated Salmonella displaying HIV-1 10E8 epitope on fimbriae: systemic and mucosal immune responses in BALB/c mice by mucosal administration." in: Scientific reports, Vol. 6, pp. 29556, (2018) (PubMed).

    Sun, Zhang, Li, Wang, Ma, Sun, Liu, Wang, Zhou: "Interaction between neurotrophin 4 and gonadotrophin in bovine oviducts." in: Theriogenology, Vol. 78, Issue 1, pp. 39-48, (2012) (PubMed).

  • 抗原 See all Neurotrophin 4 (NTF4) ELISA试剂盒
    Neurotrophin 4 (NTF4)
    别名
    NTF4 (NTF4 产品)
    别名
    NTF5 ELISA Kit, GLC10 ELISA Kit, GLC1O ELISA Kit, NT-4 ELISA Kit, NT-4/5 ELISA Kit, NT-5 ELISA Kit, NT4 ELISA Kit, NT5 ELISA Kit, NT4P ELISA Kit, Ntf5 ELISA Kit, 2900040K06Rik ELISA Kit, AI462899 ELISA Kit, NT4/5 ELISA Kit, Ntf-5 ELISA Kit, Ntf4 ELISA Kit, neurotrophin 4 ELISA Kit, neurotrophin 5 ELISA Kit, NTF4 ELISA Kit, Ntf4 ELISA Kit, ntf4 ELISA Kit, Ntf5 ELISA Kit
    背景

    Protein Function: Target-derived survival factor for peripheral sensory sympathetic neurons.

    Background: Neurotrophin-4(NT-4), also known as neurotrophin-5(NT-5), is a protein that in humans is encoded by the NTF4 gene. Human NT-4 as well as a human NT-4 pseudogene colocalize to chromosome 19 band q13.3. NT-4 is a member of a family of neurotrophic factors, the neurotrophins, that control survival and differentiation of vertebrate neurons(2-4). NT-4 is a neurotrophic factor that signals predominantly through the TrkB receptor tyrosine kinase. NT4 plays a physiological role essential for hippocampus- and amygdala-dependent long-term memory and hippocampal long-lasting LTP and that NT4 may be useful in the therapy of acquired disorders of learning and memory.

    Synonyms: Neurotrophin-4,NT-4,Neurotrophin-5,NT-5,Neutrophic factor 4,NTF4,NTF5,

    Full Gene Name: Neurotrophin-4

    Cellular Localisation: Secreted.
    基因ID
    4909
    UniProt
    P34130
    途径
    RTK signaling
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