CD200 ELISA 试剂盒
Quick Overview for CD200 ELISA 试剂盒 (ABIN1672888)
抗原
See all CD200 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CD200
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: Q31-G232 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: Q31-G232
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CD200 (CD200) ELISA Kit
CD200 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
CD200 (CD200) ELISA KitCD200 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
CD200 (CD200) ELISA KitCD200 适用: 大鼠 Colorimetric Sandwich ELISA 47 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
CD200 (CD200) ELISA KitCD200 适用: 小鼠 AA 31-232 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
CD200 (CD200) ELISA KitCD200 适用: 人 Colorimetric Sandwich ELISA 20-6000 pg/mL Cell Culture Supernatant, Plasma, Serum
CD200 (CD200) ELISA KitCD200 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
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板类型
- Pre-coated
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实验流程
- human CD200 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD200 has been precoated onto 96-well plates. Standards(NSO, Q31-G232) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD200 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD200 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CD200 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CD200 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 120, Standard deviation: 6.12, CV(%): 5.1
- Sample 2: n=16, Mean(pg/ml): 635, Standard deviation: 34.29, CV(%): 5.4
- Sample 3: n=16, Mean(pg/ml): 1149, Standard deviation: 70.09, CV(%): 6.1,
- Sample 1: n=24, Mean(pg/ml): 130, Standard deviation: 8.06, CV(%): 6.2
- Sample 2: n=24, Mean(pg/ml): 623, Standard deviation: 35.51, CV(%): 5.7
- Sample 3: n=24, Mean(pg/ml): 1208, Standard deviation: 90.6, CV(%): 7.5
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- CD200
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别名
- CD200
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背景
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Protein Function: Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.
Background: OX-2 membrane glycoprotein, also known as CD200 is a human protein encoded by the CD200 gene. This gene is mapped to 3q13.2. The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. It is suggested that CD200-CD200R signaling plays a central role in macrophage fusion and osteoclast formation.
Synonyms: OX-2 membrane glycoprotein,CD200,CD200,MOX1, MOX2,My033,
Full Gene Name: OX-2 membrane glycoprotein
Cellular Localisation: Cell membrane, Single-pass type I membrane protein. -
基因ID
- 4345
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UniProt
- P41217
抗原 See all CD200 ELISA试剂盒
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